1338例男性无精症或少精症患者Y染色体微缺失分析  

Analysis of Y Chromosome Microdeletions in 1338 Male Patients with Azoospermia or Oligozoospermia

作　　者：于海洋[1] 杨静静[2] 曾昭书[3] YU Haiyang;YANG Jingjing;ZENG Zhaoshu(Clinical Laboratory,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Reproductive Center,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Basic Medical College,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]郑州大学第三附属医院检验科,河南郑州450052 [2]郑州大学第三附属医院生殖中心,河南郑州450052 [3]郑州大学基础医学院,河南郑州450001

出　　处：《临床医学工程》2023年第2期283-284,共2页Clinical Medicine & Engineering

摘　　要：目的探讨男性无精症或少精症患者的Y染色体微缺失情况。方法1338例男性不育患者按照精液常规检查结果分为无精症组、严重少精症组、少精症组,选取同期320例健康男性为正常对照组,应用荧光定量PCR技术进行AZF区微缺失分析。结果无精症组和严重少精症组的Y染色体微缺失发生率均显著高于正常对照组(P<0.05)。在入组的研究对象中,AZFc位点缺失在男性不育中占比最高,为4.9%。在118例Y染色体微缺失患者中,共有25例染色体核型异常,其中以47,XXY核型占比最高,为8.5%。结论Y染色体微缺失检测是男性少精症或无精症等的首选临床检测项目,对男性不育的诊断有重要的指导价值。Objective To explore the Y chromosome microdeletion in male patients with azoospermia or oligozoospermia.Methods A total of 1338 male patients with infertile were divided into azoospermia group,severe oligozoospermia group and oligozoospermia group according to the results of semen routine examination,and 320 healthy men were selected as normal control group.AZF zone microdeletion analysis was performed by fluorescence quantitative PCR.Results The incidence of Y chromosome microdeletion in normal control group was lower than that in azoospermia group and severe oligozoospermia group(P<0.05).Among the subjects enrolled,the AZFc locus deletion accounted for the highest proportion(4.9%)of male infertility.Among 118 patients with Y chromosome microdeletion,25 cases had chromosomal karyotype abnormalities,of which 47,XXY karyotype accounted for the highest proportion(8.5%).Conclusions Y chromosome microdeletion detection is the preferred clinical detection project for male oligospermia or azoospermia,which has important guiding value for the diagnosis of male infertility.

关 键 词：无精症 少精症 Y染色体微缺失 不育 

分 类 号：R698.2[医药卫生—泌尿科学]