基于2型糖尿病小鼠模型探索生品黑豆、淡豆豉对二肽基肽酶-4活性的影响  

Effects of Black Soybeans and Sojae Semen Praeparatum on Activity of Dipeptidyl Peptidase Ⅳ Based on Mouse Model of Type 2 Diabetes

作　　者：刘晶[1] 黄文娟[1] 卢军[1] 张彦[1] 范芳芳[1] 赵生俊[1] LIU Jing;HUANG Wenjuan;LU Jun;ZHANG Yan;FAN Fangfang;ZHAO Shengjun(Department of Pharmacy,Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学附属中医医院药学部,乌鲁木齐830000

出　　处：《世界中医药》2023年第1期70-74,80,共6页World Chinese Medicine

基　　金：新疆医科大学附属中医医院院级课题(ZYY2018PZ1);新疆维吾尔自治区自然科学基金资助项目(2020D01C144)。

摘　　要：目的:比较生品黑豆、淡豆豉对2型糖尿病小鼠模型二肽基肽酶-4(DPP-4)活性的影响。方法:建立高糖高脂饮食联合链脲佐菌素(STZ)诱导的2型糖尿病小鼠模型,造模成功后随机分成正常对照组、模型组、阳性对照组、生品黑豆低剂量组、生品黑豆中剂量组、生品黑豆高剂量组、淡豆豉低剂量组、淡豆豉中剂量组、淡豆豉高剂量组。给药期间监测动物血糖变化情况,采用酶法检测血清中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平,用酶联免疫吸附试验测定血清中DPP-4活性、胰高血糖素样肽-1(GLP-1)、葡萄糖依赖性胰岛素释放肽(GIP)水平。结果:药物干预期间,阳性对照组和淡豆豉高剂量组血糖下降最为显著(P<0.05)。与模型组比较,淡豆豉中剂量组中仅HDL-C水平相对升高,淡豆豉高剂量组中TC、TG、LDL-C、HDL-C水平均有明显变化(P<0.05);与模型组比较,阳性对照组、生品黑豆高剂量组、淡豆豉中剂量组、淡豆豉高剂量组小鼠血清中DPP-Ⅳ活性抑制率均有升高(P<0.01),阳性对照组和淡豆豉高剂量组GLP-1、GIP水平明显高于模型组(P<0.05)。结论:淡豆豉提取物能够降低2型糖尿病小鼠血糖、血脂等相关生化指标,对2型糖尿病小鼠DPP-4活性有抑制作用,同时可以提高GLP-1、GIP水平。Objective:To compare the effects of black soybeans and Sojae Semen Praeparatum(SSP) on the activity of dipeptidyl peptidase Ⅳ(DPP-4) in the mouse model of type 2 diabetes.Methods:The model of type 2 diabetes was induced in mice by high-sugar and high-fat diet combined with streptozotocin(STZ).After modeling, the mice were randomly divided into a normal control group, a model control group, a positive control group, low-,medium-,and high-dose black soybeans groups, and low-,medium-,and high-dose SSP groups.The blood sugar changes of the mice were monitored during drug administration.The serum levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C) were detected by the enzymatic method.The DPP-4 activity, glucagon-like peptide-1(GLP-1),and glucose-dependent insulinotropic peptide(GIP) in serum were determined by ELISA.Results:The blood sugar in the positive control group and the high-dose SSP group decreased significantly during drug intervention(P<0.05).Compared with the results in the model control group, only the HDL-C level increased in the medium-dose SSP group, and the levels of TC,TG,LDL-C,and HDL-C in the high-dose SSP group changed significantly(P<0.05).Compared with the model control group, the positive control group, the high-dose black soybeans group, and medium-and high-dose SSP groups showed increased inhibition rates of DPP-Ⅳ activity in serum(P<0.01).The levels of GLP-1 and GIP in the positive control group and the high-dose SSP group were higher than those in the model control group(P<0.05).Conclusion:SSP extract can reduce blood sugar, blood fat, and other biochemical indicators in the mouse model of type 2 diabetes to varying degrees, inhibit DPP-4 activity, and increase the levels of GLP-1 and GIP.

关 键 词：淡豆豉 生品黑豆 二肽基肽酶-4 胰高血糖素样肽-1 葡萄糖依赖性胰岛素释放肽 2型糖尿病小鼠 血糖 血脂 

分 类 号：R282[医药卫生—中药学] R961.1[医药卫生—中医学]