miR-146a对电点燃癫痫发作孕鼠子代的脑发育和认知功能影响  

Effects of miR-146a on Brain Development and Cognitive Function inOffspring of Pregnant Rats with Electrical Ignition Seizures

作　　者：米尔古丽·艾麦特 不艾吉尔·艾力[1] 王成凤[1] 曼古努尔·玉山[1] 仲婷[1] 景燕[1] 木克代斯·米尔地洋[1] 李红燕[1] MIERGULI Aimaite(The People's Hospital of Xinjiang Uygur Autonomous Region/Xinjiang Clinical Medical Research Center for Stroke and Nervous System Rare Diseases,Xinjiang Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院,新疆乌鲁木齐830001

出　　处：《河北医学》2023年第2期221-226,共6页Hebei Medicine

基　　金：新疆维吾尔自治区人民医院院内项目,(编号:20200405)。

摘　　要：目的:探究miR-146a对癫痫完全点燃的孕鼠子代生长和认知功能影响。方法:将雌鼠分为对照组、模型组和miR-146a抑制剂组,每组6只。取对照组大鼠仅植入电极,取模型组大鼠植入电极,通过频率60Hz,波宽1.0ms,串长10s的恒向电流点燃癫痫,取miR-146a抑制剂组大鼠植入电极,点燃癫痫后静脉注射miR-146a抑制剂(剂量:2mg/kg),每周2次,直到分娩。雌鼠癫痫点燃成功后与雄鼠合笼,观察雌鼠怀孕情况,产仔后观察仔鼠生长过程中翻正反射与抓握反应时间,RT-PCR比较脑组织中miR-146a含量。监测仔鼠体重与体长,待仔鼠生长至84d,Morris水迷宫测试评估仔鼠认知功能。尼氏染色检测神经元状态,高尔基染色评估树突棘密度。结果:与对照组相比,模型组仔鼠miR-146a含量增加(P<0.01);与模型组相比,miR-146a抑制剂组miR-146a含量减少(P<0.01)。与对照组相比,模型组仔鼠翻正反射时间增加(P<0.001),抓握反应时间减少(P<0.01),体重与体长明显降低(P<0.001),逃避潜伏期增加(P<0.01),穿越平台次数与平台象限游泳明显减少(P<0.01),尼氏细胞数量减少,树突棘长度与密度降低(P<0.01)。与模型组相比,miR-146a抑制剂组仔鼠翻正反射时间减少(P<0.001),抓握反应时间延长(P<0.01),体重与体长明显增加(P<0.001),Morris水迷宫中逃避潜伏期明显减少(P<0.01),穿越平台次数与平台象限游泳明显增加(P<0.05),尼氏细胞数量增加且树突棘长度与密度升高(P<0.05)。结论:母体孕期癫痫完全点燃造成仔鼠脑组织中miR-146a表达增加,降低miR-146a表达能够明显促进仔鼠体格生长,促进中枢神经系统发育,提高树突棘密度与长度,改善仔鼠远期的认知功能。Objective:To investigate the effect of miR-146a on the growth and cognitive function of offspring of pregnant rats with complete ignition of epilepsy.Methods:Female rats were divided into control,model,and miR-146a inhibitor groups,with 6 rats in each group.Rats in the control group were implanted with electrodes only,and rats in the model group were implanted with electrodes.Epilepsy was ignited by the constant current with a frequency of 60Hz,wave width of 1.0ms,and string length of 10s.And the rats in the miR-146a inhibitor group were implanted with electrodes,then,epilepsy was ignited,followed by miR-146a inhibitor administration(dose:2 mg/kg),twice a week until delivery.The female rats were caged together with the male rats after successful seizure ignition,and the female rats were observed to be pregnant.After the litter was born,the timing of the roll-over reflex and grasp response was observed during the growth of the litter,and the miR-146a content in brain tissue was compared by RT-PCR.The litter was monitored for body weight and length,and after the litter was grown to 84 d,the Morris water maze test was performed to assess the cognitive function of the litter.Nissler staining was used to detect the neuronal status and Golgi staining to detect the dendritic spine density.Results:Compared with the control group,the content of miR-146a in the model group increased(P<0.01);compared with the model group,the content of miR-146a in the miR-146a inhibitor group decreased(P<0.01).Compared with the control group,the offspring rats in the model group of the righting reflex time increased(P<0.01),the grasping reaction time decreased(P<0.01),the body weight and body length reduced(P<0.001),the escape latency significantly increased(P<0.01),and the number of crossing the platform and swimming in the platform quadrant significantly decreased(P<0.01);the number of Nissl cells decreased,and the length and density of dendritic spines decreased(P<0.01).Compared with the model group,the offspring rats in the miR-146a inh

关 键 词：癫痫 MIR-146A 孕期 

分 类 号：R742.1[医药卫生—神经病学与精神病学]