补肾活血汤调控炎症小体改善大鼠椎间盘退变的实验研究  

Experimental Study on Bushen Huoxue Decoction Regulating Inflammasome in Improving Intervertebral Disc Degeneration in Rats

作　　者：李明洋 冯帅华[2] 蒋佳豪 李泽湘 王镇远 罗阳骞 杨届 吴官保[2] LI Mingyang;FENG Shuaihua;JIANG Jiahao;LI Zexiang;WANG Zhenyuan;LUO Yangqian;YANG Jie;WU Guanbao(Hunan University of Chinese Medicine,Changsha 410208,China;Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南省中医药研究院附属医院

出　　处：《中国中医骨伤科杂志》2023年第1期1-6,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：湖南省自然科学基金面上项目(2022JJ30025);长沙市自然科学基金面上项目(kq2202475);湖南省中医药科研计划项目(2021094);湖南省中医药科研计划项目(201902);湖南省中医药研究院联合基金重点项目(202016)。

摘　　要：目的:观察补肾活血汤对退变椎间盘中NLRP3/Caspase-1炎症小体的影响并探究补肾活血汤改善腰椎间盘退变的作用机制。方法:SD大鼠70只(雌雄各半),随机分为3组:Ⅰ组50只进行椎间盘退变模型建立,Ⅱ组10只作为空白组(A组),Ⅲ组10只作为假手术组(B组)。验证造模成功后,Ⅰ组取44只大鼠随机分为模型组8只(C组)、抑制剂组18只(D组,Caspase-1抑制剂灌胃)及中药组18只(E组,补肾活血汤灌胃)。各组于喂养2周后随机处死组内半数大鼠取出其L3/4至L5/6椎间盘组织,4周后将剩余大鼠全部处死取L3/4至L5/6椎间盘组织,样本行苏木精-伊红(HE)染色,Western Blot法检测NLRP3、Caspase-1蛋白表达水平,PCR检测NLRP3、Caspase-1基因表达水平,ELISA法测定IL-1β、TNF-α蛋白含量。结果:与空白组比较,模型组NLRP3、Caspase-1、IL-1β、TNF-α各项指标均显著高于空白组,差异有统计学意义(P<0.01);与模型组比较,中药组NLRP3、Caspase-1、IL-1β、TNF-α各项指标均显著低于模型组,差异有统计学意义(P<0.01)。结论:补肾活血汤可以通过抑制椎间盘内NLRP3/Caspase-1炎症小体起到治疗或延缓椎间盘退变的作用。Objective:To observe the efficacy of Bushen Huoxue decoction on NLRP3/Caspase-1 inflammasome in degenerative intervertebral discs and explore the mechanism of Bushen Huoxue decoction in improving lumbar intervertebral disc degeneration.Methods:70 SD rats(half male and half female) were randomly divided into three groups, 50 rats in group Ⅰ were used to establish the intervertebral disc degeneration model, 10 rats in group Ⅱ were used as the blank group(group A),and 10 rats in group Ⅲ were used as the sham operation group(group B).After verifying the successful modeling, 44 rats in group Ⅰ were randomly divided into model group(group C,n=8),inhibitor group(group D,Caspase-1 inhibitor gavage, n=18),and traditional Chinese medicine group(group E,Bushen Huoxue decoction gavage, n=18).After feeding for 2 weeks, half of the rats in each group were killed, and their L3/4to L5/6intervertebral disc tissues were taken out.After 4 weeks, all the remaining rats were killed, and the L3/4to L5/6intervertebral disc tissues were taken out.The samples were stained with HE,the expression levels of NLRP3 and Caspase-1 proteins were detected by Western Blot, the expression levels of NLRP3 and Caspase-1 genes were detected by PCR,and the protein contents of IL-1β,TNF-α were detected by ELISA.Results:Compared with the blank group, all the indexes of NLRP3,Caspase-1,IL-1β,TNF-α in the model group were significantly higher than those in the blank group(P<0.01).Compared with the model group, all the indexes of NLRP3,Caspase-1,IL-1β,TNF-α in the traditional Chinese medicine group were significantly lower than those in the model group(P<0.01).Conclusion:Bushen Huoxue decoction can treat or delay intervertebral disc degeneration by inhibiting NLRP3/Caspase-1 inflammasome in the intervertebral disc.

关 键 词：补肾活血汤 炎症小体 椎间盘退变 

分 类 号：R-33[医药卫生]