干旱胁迫下蛋白激发子AMEP对绿豆和红小豆生理代谢的影响  

Effects of AMEP Protein on Physiological Metabolism of Mung Bean and Adzuki Bean under Drought Stress

作　　者：王思文 刘权[2] 殷奎德[1] Wang Siwen;Liu Quan;Yin Kuide(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319,China;College of Life Sciences and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319

出　　处：《山东农业科学》2023年第1期69-74,共6页Shandong Agricultural Sciences

基　　金：黑龙江省自然科学基金项目(LH2021C064);黑龙江八一农垦大学研究生创新科研项目(YJSCX2021-Y54)。

摘　　要：AMEP蛋白作为一种新型蛋白激发子具有提高植物抗逆性的作用。为探究AMEP蛋白在干旱胁迫下对植物生理代谢的影响,本试验采用盆栽控水方法,研究其对绿豆和红小豆苗期形态特征、光合荧光参数、叶绿素含量及抗氧化酶活性的影响。结果表明:干旱胁迫(DS处理)显著抑制绿豆和红小豆幼苗生长;喷施AMEP蛋白提高干旱胁迫下绿豆和红小豆幼苗的株高、根长、根表面积和生物量,其中绿豆和红小豆的地下生物量较DS处理分别增加212.36%和89.36%,达到显著水平。干旱胁迫下喷施AMEP蛋白(ADS处理)提高绿豆和红小豆叶片的最大光化学效率(Fv/Fm)和PSⅡ潜在活性(Fv/Fo),提高叶片的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)及叶绿素含量(SPAD),其中绿豆的Pn、Gs、Tr、SPAD值较DS处理分别提高21.71%、45.38%、20.85%和17.53%,红小豆的Pn、Gs、Tr、SPAD值较DS处理分别提高37.67%、15.84%、32.89%和37.31%。干旱胁迫下喷施AMEP蛋白(ADS处理)还增加绿豆和红小豆叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,其中POD活性增幅最大,分别增加56.49%和46.08%。综上表明,喷施AMEP蛋白可显著增强干旱胁迫下绿豆和红小豆幼苗的光合作用和抗氧化能力,进而提高抗旱性。As a novel protein exciton, AMEP protein can improve plant stress resistance. In order to explore the effects of AMEP protein on plant physiological metabolism under drought stress, the potted water control method was used to study its effects on the morphological characteristics, photosynthetic fluorescence parameters, chlorophyll content and antioxidant enzyme activities of mung bean and adzuki bean. The results showed that drought stress(DS treatment) significantly inhibited the seedling growth of mung bean and adzuki bean. Spraying AMEP protein increased the plant height, root length, root surface area and biomass under drought stress, and the underground biomass of mung bean and adzuki bean significantly increased by 212.36% and 89.36%, respectively, compared with DS treatment. Spraying AMEP protein under drought stress(ADS treatment) increased the maximum photochemical efficiency(Fv/Fm), potential activity of PSⅡ(Fv/Fo) of leaves, net photosynthetic rate(Pn), stomatal conductance(Gs), transpiration rate(Tr) and chlorophyll content(SPAD), among which, the Pn, Gs, Tr and SPAD values of mung bean increased by 21.71%, 45.38%, 20.85% and 17.53%, respectively, compared with DS treatment, while those of adzuki beans increased by 37.67%, 15.84%, 32.89% and 37.31%, respectively. ADS treatment also increased the activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) in leaves, among which, POD activity increased the most by 56.49% and 46.08%. In conclusion, spraying AMEP protein could significantly enhance the photosynthesis and antioxidant capacity of mung bean and adzuki bean seedlings under drought stress, thereby improving drought resistance.

关 键 词：绿豆 红小豆 AMEP蛋白 形态特征 光合荧光参数 生理代谢 干旱 

分 类 号：S521[农业科学—作物学] S522