β-烟酰胺单核苷酸的生理活性与合成研究进展  

Advances in physiological activities and synthesis ofβ-nicotinamide mononucleotide

作　　者：陈宇娴 周楚然 黄建忠 陶勇[2] 柯崇榕 杨欣伟 CHEN Yuxian;ZHOU Churan;HUANG Jianzhong;TAO Yong;KE Chongrong;YANG Xinwei(Engineering Research Center of Industrial Microbiology,Ministry of Education,National and Local United Engineering Research Center of Industrial Microbiology and Fermentation Technology,College of Life Sciences,Fujian Normal University,Fuzhou 350108,Fujian,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]福建师范大学生命科学学院工业微生物发酵技术国家地方联合工程研究中心、工业微生物教育部工程中心,福建福州350108 [2]中国科学院微生物研究所,北京100101

出　　处：《生物工程学报》2023年第2期516-536,共21页Chinese Journal of Biotechnology

基　　金：福建省自然科学基金(2021J01170,2021J01172)。

摘　　要：烟酰胺单核苷酸(nicotinamide mononucleotide,NMN)作为辅酶Ⅰ(即NAD+)的关键前体之一,广泛存在于多种生物中,β异构体为其活性形式。研究表明β-NMN在多种生理代谢过程中起关键作用,作为抗衰老、改善退行性疾病和代谢性疾病的潜在活性物质,β-NMN的应用价值被深度发掘,实现规模化生产迫在眉睫。生物合成法因其具有高立体结构选择性、反应条件温和、副产物少等优点,成为合成β-NMN的首选方法。本文综述了β-NMN的生理活性、化学合成法和生物合成法,其中重点介绍生物合成法中涉及到的代谢途径,旨在探索利用合成生物学优化β-NMN生产策略的潜力,为β-NMN的代谢途径研究与高效生产提供理论基础。Nicotinamide mononucleotide(NMN)is one of the key precursors of coenzymeⅠ(NAD+).NMN exists widely in a variety of organisms,andβisomer is its active form.Studies have shown thatβ-NMN plays a key role in a variety of physiological and metabolic processes.As a potential active substance in anti-aging and improving degenerative and metabolic diseases,the application value ofβ-NMN has been deeply explored,and it is imminent to achieve large-scale production.Biosynthesis has become the preferred method to synthesizeβ-NMN because of its high stereoselectivity,mild reaction conditions,and fewer by-products.This paper reviews the physiological activity,chemical synthesis as well as biosynthesis ofβ-NMN,highlighting the metabolic pathways involved in biosynthesis.This review aims to explore the potential of improving the production strategy ofβ-NMN by using synthetic biology and provide a theoretical basis for the research of metabolic pathways as well as efficient production ofβ-NMN.

关 键 词：β-烟酰胺单核苷酸 烟酰胺核糖 磷酸核糖焦磷酸 化学合成 生物合成 

分 类 号：TQ426.97[化学工程] TQ920.6[轻工技术与工程—发酵工程]