饲料中添加姜黄素对杂交鲟幼鱼代谢酶、抗氧化和肠道结构的影响  

Effect of curcumin addition to feed on metabolic enzymes,antioxidant,and gut structure in hybrid sturgeon(Acipenser baeri♂×Acipenser schrenckii♀)

作　　者：董凯玥 王艺 陈细华[1] 刘伟[1] 褚志鹏[1] 岳华梅[1] 李创举[1] DONG Kaiyue;WANG Yi;CHEN Xihua;LIU Wei;CHU Zhipeng;YUE Huamei;LI Chuangju(Key Laboratory of Freshwater Biodiversity Conservation,Ministry of Agriculture and Rural Affairs of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;College of Animal Science,Yangtze University,Jingzhou 434100,China)

机构地区：[1]中国水产科学研究院长江水产研究所,农业农村部淡水生物多样性保护重点实验室,湖北武汉430223 [2]上海海洋大学水产与生命学院,上海201306 [3]长江大学动物科学学院,湖北荆州434100

出　　处：《中国水产科学》2023年第1期37-47,共11页Journal of Fishery Sciences of China

基　　金：现代农业产业技术体系专项(CARS-46);中央级公益性科研院所基本科研业务费专项(YFI20220802).

摘　　要：为评价饲料中添加不同水平的姜黄素对杂交鲟(Acipenserbaeri♂×Acipenserschrenckii♀)幼鱼生长和形体指标、血清生化、代谢酶活性、抗氧化功能和肠道结构的影响,在基础饲料中分别添加0、0.025 g/kg、0.05 g/kg、0.1 g/kg和0.2 g/kg的姜黄素配制成5种饲料,投喂初始体重(16.24±0.11)g的杂交鲟幼鱼。实验分为5组,每组3个重复,每个重复20尾鱼。结果显示:饲料中添加姜黄素对杂交鲟幼鱼生长没有产生显著影响。血清总胆固醇(TG)含量在姜黄素添加量为0.2 g/kg时显著降低(P<0.05)。肝谷丙转氨酶(ALT)活性在0.05 g/kg姜黄素组明显降低,谷草转氨酶(AST)活性在0.05 g/kg、0.1 g/kg、0.2 g/kg添加量时活性显著降低(P<0.05)。十二指肠蛋白酶和淀粉酶分别在0.05g/kg和0.1g/kg姜黄素组活性显著升高(P<0.05)。血清丙二醛(MDA)含量在0.025g/kg时显著降低(P<0.05),过氧化氢酶(CAT)活性在0.025g/kg时显著高于对照组(P<0.05)。随着姜黄素的添加,杂交鲟幼鱼肠道结构有所改善。综上所述,饲料中添加姜黄素能改善杂交鲟幼鱼的健康状态,对鱼体具有降脂、护肝、促进消化和提高抗氧化能力等作用,姜黄素的建议添加量为0.025~0.05 g/kg。Curcumin is a natural yellow pigment derived from turmeric and tulip rhizome,has been widely used as feed additives to improve the health of cultured animals due to its highly biologically active and functions of anti-inflammatory,antioxidant,and blood lipid-lowering.However,the application of curcumin is rarely studied in sturgeon,which is intensively cultured because of the high value of nutritional and economic.Therefore,to investigate the effect of curcumin on the growth and health of sturgeon,a 6-week feeding trial was conducted using hybrid sturgeon(Acipenser baeri♂×Acipenser schrenckii♀).A total of 300 juvenile hybrid sturgeon with an initial weight of(16.24±0.11)g were assigned to diets containing 0 g/kg,0.025 g/kg,0.05 g/kg,0.1 g/kg and 0.2 g/kg curcumin in triplicated.The results showed that the addition of curcumin in the feed had no significant effect on the growth performance and body composition of juvenile hybrid sturgeon.Curcumin addition significantly decreased serum TG content in the group of 0.2 g/kg curcumin in diets(P<0.05),ALT and AST activities in hybrid sturgeon liver were reduced with the curcumin level increasing,which appeared significant difference with control at the curcumin level at 0.2 g/kg and 0.05 g/kg,respectively(P<0.05).Curcumin significantly improved the activity of protease in duodenum with curcumin level increasing at first,which showed the highest activity at 0.05 g/kg of curcumin supplementation respectively(P<0.05),and then decreased with curcumin further increasing.Curcumin addition improved the activity ofα-AMY significantly at 0.1 g/kg of curcumin supplementation respectively(P<0.05),and then decreased.The results of antioxidants showed that serum MDA content decreased significantly in the group of 0.025 g/kg curcumin(P<0.05),in which group serum CAT activity increased significantly(P<0.05).While a significant increase of CAT activity in the liver appeared in the curcumin addition level at 0.05 g/kg(P<0.05).There was no significant difference among the treatmen

关 键 词：杂交鲟 姜黄素 代谢酶 抗氧化功能 

分 类 号：S961[农业科学—水产养殖]