单细胞转录组测序技术在胃癌研究中的应用  

Application of single-cell transcriptome sequencing in gastric cancer

作　　者：张鑫 王艳春[1] 刘纯杰 Xin Zhang;Yan-Chun Wang;Chun-Jie Liu(Institute of Biotechnology,Academy of Military Medical Sciences,Beijing 100071,China;Department of Pharmacy,Medical Supplies Center,Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]军事医学研究院生物工程研究所,北京市100071 [2]解放军总医院医疗保障中心药剂科,北京市100853

出　　处：《世界华人消化杂志》2023年第2期48-55,共8页World Chinese Journal of Digestology

基　　金：“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项课题,No.2018ZX10101003-005-005。

摘　　要：胃癌(gastric cancer,GC)发病率居全球常见癌症第五位,死亡率居第三位.随着单细胞RNA测序(single-cell RNA-sequencing,scRNA-seq)技术的发展,人们对GC的研究已经开始逐渐从组织病理学水平发展到单细胞转录水平.本文对scRNA-seq技术原理及其在GC研究中的应用,包括其在揭示GC癌前病变的转录特征与起源、原发性肿瘤的瘤内异质性、肿瘤微环境和转移性播散等方面的应用进行了综述.Gastric cancer(GC)is the fifth most common cancer worldwide and the third leading cause of cancer death.With the development of single-cell RNA-sequencing(sc RNA-seq)technology,the research on GC has gradually developed from the histopathological level to the transcriptional level.In this paper,we discuss the principle of scRNA-seq technology and its application in GC research,including the transcriptional characteristics and origin of GC precancerous lesions,intratumor heterogeneity of primary tumors,tumor microenvironment,and metastatic dissemination.

关 键 词：胃癌 单细胞转录组测序 癌前病变 肿瘤异质性 肿瘤微环境 肿瘤转移 

分 类 号：R735.2[医药卫生—肿瘤]