低浓度茉莉酸甲酯对烟草长柄腺毛发生发育的影响  

Effects of Low Concentration Methyl Jasmonate on Initiation and Development of Long-stalk Glandular Trichomes in Tobacco

作　　者：段史江 陈小龙 吴飞跃 王占伟 程小强 张子颖 李亚纯 谢天琪 韩助君 杨永锋 DUAN Shijiang;CHEN Xiaolong;WU Feiyue;WANG Zhanwei;CHENG Xiaoqiang;ZHANG Ziying;LI Yachun;XIE Tianqi;HAN Zhujun;YANG Yongfeng(Jiangxi Province Ji’an Branch Company,China National Tobacco Corporation,Ji’an,Fujian 343009,China;China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450016,China;China Tobacco Fujian Industrial Co.,Ltd.,Xiamen 361000,China)

机构地区：[1]江西省烟草公司吉安市公司,江西吉安343009 [2]河南中烟工业有限责任公司,郑州450016 [3]福建中烟工业有限责任公司,厦门361000

出　　处：《中国烟草科学》2022年第6期17-24,共8页Chinese Tobacco Science

基　　金：江西省烟草公司吉安市公司资助项目(202136080027195)。

摘　　要：茉莉酸甲酯(MeJA)对烟草长柄腺毛的发生具有强烈的诱导作用,但浓度过高时会抑制烟苗发育。本文以云烟87幼苗为材料,研究低浓度MeJA对腺毛发育和腺毛分泌物的影响,并利用qRT-PCR技术研究腺毛发生发育调控基因对MeJA的应答模式。结果表明,随着MeJA喷施浓度的升高,长柄腺毛的密度和腺头直径逐渐增加;喷施300μmol/L MeJA对长柄腺毛发生和发育有显著的诱导作用,且对叶片发育无明显影响;当MeJA喷施浓度达到600μmol/L时,叶片发育受到抑制。叶面化学成分分析发现,与未喷施MeJA的对照相比,喷施300μmol/L MeJA后西柏烷二萜、烷烃和蔗糖酯含量增加约一倍。基因应答模式研究发现,西柏烷二萜合成基因CBTS和CYP71D16,以及酯转运蛋白基因Nt LTP1受MeJA诱导表达;烟草腺毛发生调控基因ARF3、GIS、CD2、Wo和Cyc B2对MeJA处理均具有应答反应,其中ARF3和GIS的表达受到MeJA诱导,而CD2、Wo和Cyc B2的表达受到MeJA抑制。综上,300μmol/L MeJA可以调控腺毛发育相关基因的表达,安全、有效地促进长柄腺毛发生发育以及腺毛分泌物的合成。Methyl jasmonate(MeJA) has a strong induction effect on the initiation of long-stalk glandular trichomes in tobacco, but the development of tobacco seedlings can be inhibited by high concentration MeJA. To study the effects of low concentration MeJA on the development of glandular trichomes and trichome secretions, and to explore the responsive patterns of related genes,Nicotiana tabacum cv. Yunyan 87 seedlings at three-leaf stage were separately sprayed with MeJA at different concentrations. The results showed that the density and glandular head of long-stalk glandular trichomes increased gradually with the increase of MeJA concentrations. Among them, 300 μmol/L MeJA had a significant effect on induction of long-stalk glandular trichomes, and had no effect on leaf development. When treated with high concentration MeJA(600 μmol/L MeJA), the growth and development of leaves were inhibited. Analysis of the chemical components on leaf surface showed that the contents of cembranoids, alkanes and sucrose esters were nearly doubled after sprayed with 300 μmol/L MeJA. qRT-PCR was used to study the gene expression pattern after MeJA treatments. It was found that the key genes related to cembranoid biosynthesis CBTS and CYP71D16, as well as the lipid transporter gene NtLTP1, were activated by MeJA treatments. The expressions of ARF3, GIS, CD2, Wo and Cyc B2, which may regulate the initiation of glandular trichomes, were responsive to MeJA treatments. Among them, ARF3 and GIS were induced by MeJA, while CD2, Wo and Cyc B2 were repressed by MeJA. These results indicated that 300 μmol/L MeJA can regulate the expression of genes related to glandular trichome development, and thus safely and effectively promote the initiation and development of long-stalk glandular trichomes and trichome secretion synthesis.

关 键 词：烟草 腺毛 茉莉酸甲酯 西柏烷二萜 蔗糖酯 基因表达 

分 类 号：S572.01[农业科学—烟草工业]