响应面法优化卡氏芽孢杆菌ST-1产芽孢发酵培养基  

Optimization of fermentation medium for spore production by Bacillus cabrialesii ST-1 with response surface method

作　　者：李敏 黄满 邱炜玥 李焕梅 贾子民 龙同 赵锦芳 LI Min;HUANG Man;QIU Weiyue;LI Huanmei;JIA Zimin;LONG Tong;ZHAO Jinfang(Key Laboratory of Fermentation Engineering(Ministry of Education),School of Biological Engineering and Food,Hubei University of Technology,Wuhan 430068,China;Biopesticide Engineering Research Center of Hubei Province,Wuhan 430064,China)

机构地区：[1]湖北工业大学生物工程与食品学院发酵工程教育部重点实验室,湖北武汉430068 [2]湖北省生物农药工程研究中心,湖北武汉430064

出　　处：《中国酿造》2023年第2期193-198,共6页China Brewing

基　　金：湖北省教育厅重点项目(D20201403);农业部华中作物有害生物综合治理重点实验室开放基金项目(2019ZTSJJ3);湖北工业大学大学生创新创业项目(S202110500049)。

摘　　要：以卡氏芽孢杆菌(Bacillus cabrialesii)ST-1为研究对象,芽孢数为响应值,采用单因素试验、Plackett-Burman试验、最陡爬坡试验及响应面试验对其发酵培养基进行优化。结果表明,影响卡氏芽孢杆菌ST-1芽孢数的主要因素为蔗糖、麸皮和蛋白胨、磷酸二氢钾,卡氏芽孢杆菌ST-1产芽孢的最适宜培养基配方为:蔗糖13.1 g/L,麸皮+蛋白胨(1∶2)17.0 g/L,磷酸二氢钾2.0 g/L。在此最优条件下,卡氏芽孢杆菌ST-1发酵液中芽孢数达到5.96×10^(9) CFU/mL,是优化前的32.21倍。Using Bacillus cabrialesii ST-1 as the research object,and spore number as the response value,the fermentation medium was optimized by single factor tests,Plackett-Burman tests,steepest climb tests,and response surface tests.The results showed that the main factors affecting the spore number of B.cabrialesii ST-1 were sucrose,bran and peptone,and potassium dihydrogen phosphate.The optimal medium formula for spore production by B.cabrialesii ST-1 was as follows:sucrose 13.1 g/L,bran+peptone(1∶2)17.0 g/L,potassium dihydrogen phosphate 2.0 g/L.Under the optimal conditions,the spore number in the fermentation broth of B.cartesii ST-1 reached 5.96×10^(9) CFU/ml,which was 32.21 times that of before optimization.

关 键 词：卡氏芽孢杆菌 芽孢数 培养基优化 响应面法 

分 类 号：S816.7[农业科学—饲料科学]