FABP2基因在不同比例花椒籽饲喂育肥猪的组织差异表达、克隆及生物信息学分析  

Differential Expression,Cloning and Bioinformatics of FABP2 Gene in Tissues of Fattening Pigs Fed with Different Proportions of Zanthoxylum Seeds

作　　者：李杰[1] 黄晓宇[1] 尚学峰 杨姣姣[1] 张娟丽 谢开会 张亚丽 闫尊强 王鹏飞[1] 高小莉[1] 杨巧丽 马艳萍[2] 滚双宝[1,3] LI Jie;HUANG Xiaoyu;SHANG Xuefeng;YANG Jiaojiao;ZHANG Juanli;XIE Kaihui;ZHANG Yali;YAN Zunqiang;WANG Pengfei;GAO Xiaoli;YANG Qiaoli;MA Yanping;GUN Shuangbao(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Gansu Agricultural University Library,Lanzhou 730070,China;Gansu Research Center for Swine Production Engineering and Technology,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]甘肃农业大学图书馆,甘肃兰州730070 [3]甘肃省现代养猪技术工程研究中心,甘肃兰州730070

出　　处：《华北农学报》2022年第6期228-238,共11页Acta Agriculturae Boreali-Sinica

基　　金：甘肃省科技创新项目(GSSYLXM-02);甘肃省青年基金项目(20JR5RA005)。

摘　　要：旨在分析不同比例花椒籽替代部分玉米饲喂三元杂交育肥猪不同组织FABP2的差异表达,寻找肌内脂肪沉积的关键基因;同时克隆三元杂交育肥猪FABP2编码区序列,并用生物信息学方法对其进行分析。选取288头90日龄杜×长×大三元杂交育肥猪(33 kg),随机分为4组,每组4个重复,每个重复18头猪;对照组饲喂基础日粮,试验组分别用花椒籽代替饲粮中2.5%(试验Ⅰ组),5.0%(试验Ⅱ组),7.5%(试验Ⅲ组)的玉米,预试验7 d,正试期100 d。采用qRT-PCR检测试验组不同组织中FABP2的相对表达量,同时克隆三元杂交育肥猪FABP2编码区,利用生物信息学分析软件,预测FABP2蛋白理化性质、结构域和磷酸化激酶位点等。结果表明:FABP2在各组织中均有不同程度表达,其中空肠和肝脏中FABP2显著高表达。与对照组相比,各试验组心脏、肺脏、肾脏、十二指肠、空肠和盲肠组织中FABP2显著下调表达;试验Ⅱ组和试验Ⅲ组脾脏和回肠组织中FABP2显著上调表达;试验Ⅰ组肝脏、结肠、直肠和背肌组织中FABP2显著上调表达,说明饲粮中添加花椒籽对三元杂交育肥猪FABP2基因的表达谱具有显著影响。成功克隆并鉴定出三元杂交育肥猪FABP2编码区全长(399 bp),编码132个氨基酸,存在2处错义突变;FABP2蛋白是一种酸性稳定的非分泌蛋白,二级结构主要由延伸链和无规则卷曲构成,含有一个Lipocalin-FABP2超家族保守结构域和3个无序化区域。The purpose of this study was to analyze the differential expression of FABP2 in different tissues of DLY fattening pigs fed different proportions of Zanthoxylum seeds instead of some corn,and to find the key gene for intramuscular fat deposition;at the same time,the sequence of the coding region of FABP2 in DLY fattening pigs was cloned and analyzed by bioinformatics methods.A total of 288,90-day-old Duroc Landrace Yorkshire fattening pigs(33 kg)were randomly divided into 4 groups with 4 replicates and 18 pigs in each replicate;the control group was fed the basal diet and the test group was fed Zanthoxylum seeds instead of corn in the diet at 2.5%(test groupⅠ),5.0%(test groupⅡ)and 7.5%(test groupⅢ),respectively,for 7 d in the pretest and 100 d in the test period.PCR was used to detect the relative expression of FABP2 in different tissues of the test groups,and the coding region of FABP2 in DLY fattening pigs was also cloned,and bioinformatics analysis software was used to predict the physicochemical properties,structural domains and phosphorylation kinase sites of FABP2 protein.The results showed that FABP2 was differentially expressed in all tissues,with significantly higher expression in jejunum and liver.Compared with the control group,FABP2 was significantly down-regulated in heart,lung,kidney,duodenum,jejunum and cecum tissues in each test group;FABP2 was significantly up-regulated in spleen and ileum tissues in test groupⅡand test groupⅢ;FABP2 was significantly up-regulated in liver,colon,rectum and dorsal muscle tissues in test groupⅠ,indicating that the addition of Zanthoxylum seeds in the diet had a significant effect on the expression profile of FABP2 gene in DLY fattening pigs.In this experiment,the full length(399 bp)of the coding region of FABP2 was successfully cloned and identified,encoding 132 amino acids,with two missense mutations;FABP2 protein was an acidic stable non-secretory protein with a secondary structure consisting mainly of extended chains and random coils,containing a Lip

关 键 词：三元杂交育肥猪 花椒籽 FABP2基因 组织表达 克隆 生物信息学分析 

分 类 号：S828.03[农业科学—畜牧学] Q78[农业科学—畜牧兽医]