超高效液相色谱法定量检测干果中20种酸性染料  

Simultaneous determination of 20 acid dyes by ultra-high performance liquid chromatography in dried fruits

作　　者：杨涛[1,2] 刘俊[3] 刘卫国 粟有志[4] 常要宝 毛雨晴 YANG Tao;LIU Jun;LIU Weiguo;SU Youzhi;CHANG Yaobao;MAO Yuqing(College of Ecology and Environment,Xinjiang University,Urumqi 830017,China;Key Laboratory of Oasis Ecology of Education Ministry,Urumqi 830017,China;Chengdu Customs Technology Center,Chengdu 610041,China;Technical Center of Yining Customs,Yining 835099,China)

机构地区：[1]新疆大学生态与环境学院,新疆乌鲁木齐830017 [2]绿洲生态教育部重点实验室,新疆乌鲁木齐830017 [3]成都海关技术中心,四川成都610041 [4]伊宁海关技术中心,新疆伊宁835099

出　　处：《食品与发酵工业》2023年第4期245-249,共5页Food and Fermentation Industries

基　　金：新疆维吾尔自治区自然科学基金(2020D01A126)。

摘　　要：该研究建立了超高效液相色谱-二极管阵列检测器法测定干果中20种酸性染料的方法。方法采用乙腈-水(体积比17∶3)为提取溶剂,提取液经凝胶色谱柱净化,以及选用C18柱进行分离,经二极管阵列检测器进行目标痕量水平检测。结果表明,该方法具有良好的线性范围(10~50μg/mL),相关系数(r)大于0.9883,检测限为0.01~0.26μg/g,回收率为71%~114%,相对标准偏差(n=6)为0.80%~7.5%。经检验,该方法具有高灵敏度和可靠性,可同时检测食品中的20种酸性染料。A simultaneous determination method for 20 acid dyes in food was developed by ultra-high performance liquid chromatography equipped with a diode array detector.The samples were extracted with an ultrasonic-assisted solution of acetonitrile water(17∶3,volume ratio).The clear supernatant extract was purified by the GPC cleanup system.The obtained eluate was separated by the C18 column and monitored by a diode array detector.Under this condition,this study got good linearity in the range of 10-50μg/mL with correlation coefficients above r>0.9883.The limit of detection and recovery of the method was in the ranges of 0.01-0.26μg/g and 71%-114%.The precision was 0.80%-7.5%(n=6).This method is a convenient,high-sensitivity,and low-cost method for simultaneously determining 20 acid dyes in foodstuffs.

关 键 词：超高效液相色谱法 二极管阵列检测器 酸性染料 凝胶色谱柱 干果 

分 类 号：O657.72[理学—分析化学] TS255.7[理学—化学]