糖基转移酶基因TaUGT7分子表达特征及抗赤霉病作用分析  

Molecular characterization of a glycosyltransferase gene TaUGT7 and its resistance to Fusarium head blight

作　　者：何漪 刘祥 吴磊[1] 姜朋[1] 马鸿翔 张旭[1] He Yi;Liu Xiang;Wu Lei;Jiang Peng;Ma Hongxiang;Zhang Xu(CIMMYT-JAAS Joint Center for Wheat Diseases,Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu Province,China;School of Life Sciences,Jiangsu University,Zhenjiang 212013,Jiangsu Province,China;Jiangsu Provincial Key Laboratory of Crop Genomics and Molecular Breeding,Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Agricultural College,Yangzhou University,Yangzhou 225009,Jiangsu Province,China)

机构地区：[1]江苏省农业科学院粮食作物研究所,CIMMYT-JAAS小麦病害联合研究中心,南京210014 [2]江苏大学生命科学技术学院,镇江212013 [3]扬州大学农学院,江苏省粮食作物现代产业技术协同创新中心,江苏省作物基因组学与分子育种重点实验室,扬州225009

出　　处：《植物保护学报》2023年第1期197-205,共9页Journal of Plant Protection

基　　金：国家重点研发计划(2017YFE0126700,2016YFD0101802);国家自然科学基金(31801727,31561143004)。

摘　　要：为研究小麦UDP-葡萄糖基转移酶7(UDP-glycosyltransferase 7,TaUGT7)的抗赤霉病功能,利用DNAMAN 6.0软件对Ta UGT7及其同源蛋白进行序列比对,应用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术分析经赤霉菌Fusarium graminearum和脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)处理后的苏麦3号小穗中TaUGT7基因的表达特征,利用基因枪在洋葱表皮细胞瞬时表达TaUGT7-eGFP进行亚细胞定位,采用农杆菌介导法在小麦品种Fielder中过量表达TaUGT7基因并进行赤霉病抗性鉴定。结果表明,TaUGT7在氨基酸序列上与已知赤霉病抗性相关UGT相似性较低;TaUGT7在赤霉菌接种24 h后开始被诱导表达,在DON处理2 h后逐步被诱导表达;Ta UGT7蛋白亚细胞定位于细胞膜和细胞核中;qRT-PCR检测发现,TaUGT7在8株独立的过表达转基因株系中均有不同程度的上调表达;与野生型对照相比,过表达株系TaUGT7-395和TaUGT7-457中的平均病小穗率显著下降。表明TaUGT7可以被赤霉菌和DON诱导表达,并在小麦抗赤霉病过程中发挥作用。To clarify the function of UDP-glycosyltransferase 7(TaUGT7)in resistance to Fusarium head blight(FHB)in wheat,homologous analysis was conducted using DNAMAN 6.0 software,and the expression patterns of TaUGT7 in wheat spikelets were analyzed with quantitative real-time PCR(qRT-PCR)in response to Fusarium graminearum and deoxynivalenol(DON).The TaUGT7-eGFP was delivered into onion epidermal cells via gene gun bombardment to determine its subcellular localization.TaUGT7 gene was overexpressed in wheat variety Fielder using the Agrobacterium-mediated method to investigate its role in resistance to FHB.The results showed that TaUGT7 shared a low similarity with those UGT proteins previously reported to contribute to FHB resistance at the amino acid level.TaUGT7 was distributed throughout cells including cell membrane and nuclei.Eight independent transgenic overexpression lines were obtained,all of which were up-regulated in different degrees by qRTPCR detection.In compared with the wild-type control,the proportion of symptomatic spikelets decreased significantly in the overexpression lines TaUGT7-395 and TaUGT7-457.Transcript of TaUGT7was induced after F.graminearum or DON inoculation,and TaUGT7 overexpression in wheat showed improved resistance to Fusarium spread.

关 键 词：小麦 赤霉病 UDP-葡萄糖基转移酶 脱氧雪腐镰刀菌烯醇 

分 类 号：S435.121.45[农业科学—农业昆虫与害虫防治]