PD1-TCF1+CD8+干细胞样记忆T细胞对肿瘤患者免疫预后及三级淋巴结构的影响  

Effect of PD1-TCF1+CD8+Stem-like Memory T Cells on Immunotherapy Prognosis and Tertiary Lymphoid Structures in Tumors

作　　者：崔雪 翁一鸣[1] 王培伟 彭敏[1] CUI Xue;WENG Yiming;WANG Peiwei;PENG Min(Department of Oncology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院肿瘤中心,武汉430060

出　　处：《肿瘤防治研究》2023年第2期146-150,共5页Cancer Research on Prevention and Treatment

基　　金：吴阶平医学基金会课题(320.6750.2021-02-127);北京科创医学发展基金会课题(KC2021-JX-0186-18)。

摘　　要：目的探讨PD1-TCF1+CD8+干细胞样记忆T细胞在肿瘤微环境中的表达及对免疫治疗预后及三级淋巴结构(TLSs)的影响。方法收集33例武汉大学人民医院接受免疫治疗的晚期胸部肿瘤患者病理组织切片,18例NSCLC及15例食管鳞癌(ESCC)。多重免疫荧光技术对PD1-TCF1+CD8+T细胞表型进行定量分析,Kaplan-Meier法绘制生存曲线,Pearson检验进行相关性分析。结果PD1-TCF1+CD8+T细胞的高表达与更好的无进展生存期(PFS)和免疫治疗疗效有关;在NSCLC队列中,PD1-TCF1+CD8+T细胞的高表达还与三级淋巴结构的数量(P=0.0017)及相对面积(P=0.006)显著正相关。结论在NSCLC和ESCC患者中,PD1-TCF1+CD8+干细胞样记忆T细胞的高表达可能与良好的预后及免疫治疗疗效有关,并且在NSCLC中与肿瘤微环境内TLSs的形成密切相关。Objective To investigate the impact of PD1-TCF1+CD8+stem-like memory T cells on immunotherapy prognosis and tertiary lymphoid structure in tumors.Methods Pathological tissue sections were collected from 33 patients treated with immunotherapy,18 cases of NSCLC and 15 cases of ESCC.The expression of PD1-TCF1+CD8+T cells was detected through quantitative analysis by multiplex immunofluorescence.Survival curves were described by the Kaplan-Meier method.Pearson’s correlation test was used for correlation analysis.Results The high levels of PD1-TCF1+CD8+T cells had a better PFS in NSCLC and ESCC cohorts.In the NSCLC cohort,high levels of PD1-TCF1+CD8+T cells were significantly and positively correlated with the number(P=0.0151)and size(P=0.0007)of TLSs.Conclusion In patients with NSCLC and ESCCs,high PD1-TCF1+CD8+stem-like memory T cell expression indicates improved prognosis and immune response and is associated with the formation of TLSs in the tumor microenvironment of NSCLC.

关 键 词：肿瘤微环境 预后 三级淋巴结构 干细胞样记忆T细胞 

分 类 号：R730.45[医药卫生—肿瘤]