长链非编码RNA肿瘤蛋白翻译调节因子1-反义RNA1在神经胶质瘤细胞中的表达及对其增殖和侵袭的影响  

Expression of long non-coding RNA tumor protein translationally-controlled regulator 1-antisenseRNA1 in glioma and its effect on cell proliferation and invasion

作　　者：刘振杰[1] 李鑫[1] 鲁静 梁良 胡耀文 解靖 赵新岗[3] 杨建凯[4] LIU Zhenjie;LI Xin;LU Jing;LIANG Liang;HU Yaowen;XIE Jing;ZHAO Xingang;YANG Jiankai(Department of Neurosurgery,Baoding NO.1 Central Hospital,Hebei Province,Baoding071000,China;Department of Internal Medicine,Baoding Orthopaedic Hospital,Hebei Province,Baoding071000,China;Department of Neurosurgery,Sanbo Brain Hospital,Capital Medical University,Beijing100093,China;Department of Neurosurgery,Second Hospital of Hebei Medical University,Hebei Province,Shijiazhuang050000,China)

机构地区：[1]河北省保定市第一中心医院神经外科,河北保定071000 [2]河北省保定骨科医院内科,河北保定071000 [3]首都医科大学三博脑科医院神经外科,北京100093 [4]河北医科大学第二医院神经外科,河北石家市050000

出　　处：《中国医药导报》2023年第4期25-30,共6页China Medical Herald

基　　金：河北省高层次人才资助项目(A202103004);河北省保定市科技计划项目(2041ZF073)。

摘　　要：目的探讨长链非编码RNA(lncRNA)肿瘤蛋白翻译调节因子1-反义RNA1(TPT1-AS1)对神经胶质瘤细胞U251增殖、侵袭的影响。方法将U251分为空白对照组、阴性转染组、TPT1-AS1过表达组,空白对照组不做处理,另两组进行相应质粒的转染,转染48 h后比较各组U251细胞中TPT1-AS1表达水平;MTT法测定各组U251细胞的存活率;流式细胞仪测定各组U251细胞的凋亡能力;Transwell小室实验检测各组U251细胞侵袭能力;划痕实验检测各组U251细胞迁移能力;Western blot检测各组U251细胞凋亡[半胱氨酸蛋白酶3(Caspase-3)、Bax、Bcl-2]及侵袭[基质金属蛋白酶(MMP)-2、MMP-9]相关蛋白表达情况。结果阴性对照组与空白对照组比较,TPT1-AS1表达、细胞存活率、细胞凋亡率、侵袭细胞数、划痕愈合率及Caspase-3、Bax、Bcl-2、MMP-2、MMP-9蛋白表达,差异无统计学意义(P>0.05)。TPT1-AS1过表达组TPT1-AS1表达水平、细胞凋亡能力、Caspase-3、Bax蛋白表达均高于空白对照组,差异有统计学意义(P<0.05);TPT1-AS1过表达组Bcl-2蛋白表达、细胞存活率、侵袭、迁移能力、侵袭相关蛋白表达均低于空白对照组,差异有统计学意义(P<0.05)。结论TPT1-AS1在U251中呈现低表达,高表达的TPT1-AS1可抑制神经胶质瘤细胞的增殖及侵袭能力。Objective To investigate the effect of long non-coding RNA(lncRNA)tumor protein translation regulator 1-antisense RNA1(TPT1-AS1)on the proliferation and invasion of glioma cells U251.Methods U251 cells were divided into blank control group,negative transfection group,and TPT1-AS1 overexpression group.The blank control group was not treated,and the other two groups were transfected with corresponding plasmids.After 48 hours of transfection,the expression level of TPT1-AS1 in U251 cells of each group was compared;MTT assay was used to determine the proliferation ability of U251 cells;flow cytometry was used to detect the apoptotic ability of U251 cells;Transwell chamber assay was used to detect the invasion ability of U251 cells;scratch test was used to detect the migration ability of U251 cells;the expression of apoptosis(Caspase-3,Bax,Bcl-2)and invasion(matrix metallo proteinases[MMP]-2,MMP-9)related proteins was detected by Western blot.Results There were no significant differences in TPT1-AS1 expression,cell survival rate,cell apoptosis rate,number of invasive cells,scratch healing rate,and protein expressions of Caspase-3,Bax,Bcl-2,MMP-2,and MMP-9 between negative control group and blank control group(P>0.05).TPT1-AS1 expression level,apoptosis capacity,Caspase-3,and Bax protein expression in TPT1-AS1 overexpression group were higher than those in blank control group,and the differences were statistically significant(P<0.05).The expression of Bcl-2 protein,cell survival rate,invasion,migration ability,and invasion-related protein in TPT1-AS1 overexpression group were lower than those in blank control group,with statistical significance(P<0.05).Conclusion TPT1-AS1 has low expression in U251,and high expression of TPT1-AS1 can inhibit the proliferation and invasion of glioma cells.

关 键 词：长链非编码RNA肿瘤蛋白翻译调节因子1-反义RNA1 神经胶质瘤 增殖 侵袭 

分 类 号：R73[医药卫生—肿瘤]