子宫肌瘤组织中miR-23、HIF-1α表达水平及其与细胞侵袭、上皮间质转化的相关性  

Expression levels of miR-23 and HIF-1α in uterine fibroids tissue and their correlation with cell invasion and epithelial mesenchymal transformation

作　　者：曹伟 CAO Wei(Department of Obstetrics and Gynecology,Nanchang County People′s Hospital,Nanchang,Jiangxi 330200,China)

机构地区：[1]江西省南昌市南昌县人民医院妇产科,江西南昌330200

出　　处：《检验医学与临床》2023年第5期645-649,共5页Laboratory Medicine and Clinic

摘　　要：目的探讨子宫肌瘤组织中微小RNA-23(miR-23)、低氧诱导因子-1α(HIF-1α)表达水平及其与细胞侵袭、上皮间质转化(EMT)的相关性。方法选取2021年1月至2022年1月于该院行手术切除治疗的子宫肌瘤患者84例为研究对象,收集其切除的子宫肌瘤组织和正常肌层组织(距子宫肌瘤0.5 cm以上)进行研究。比较子宫肌瘤组织及正常肌层组织miR-23、HIF-1αmRNA表达水平,雌激素受体(ER)、孕激素受体(PR)阳性率,以及增殖基因[B淋巴细胞瘤-2(Bcl-2)]、凋亡基因[Bcl-2相关X蛋白(Bax)]、侵袭基因[基质金属蛋白酶(MMP)2、MMP9]、EMT标志基因[N-钙黏蛋白(N-cadherin)、E-钙黏蛋白(E-cadherin)]表达水平;分析子宫肌瘤组织miR-23、HIF-1αmRNA表达水平与增殖基因、凋亡基因、侵袭基因、EMT标志基因表达水平的相关性。结果子宫肌瘤组织miR-23、Bax、E-cadherin表达水平低于正常肌层组织,HIF-1αmRNA、Bcl-2、MMP2、MMP9、N-cadherin表达水平高于正常肌层组织,ER、PR阳性率高于正常肌层组织,差异有统计学意义(P<0.05)。ER、PR阳性子宫肌瘤组织miR-23表达水平分别低于ER、PR阴性子宫肌瘤组织,HIF-1αmRNA表达水平分别高于ER、PR阴性子宫肌瘤组织,差异有统计学意义(P<0.05)。子宫肌瘤组织miR-23表达水平与Bcl-2、MMP2、MMP9、N-cadherin表达水平呈负相关(P<0.05),与Bax、E-cadherin表达水平呈正相关(P<0.05);子宫肌瘤组织HIF-1αmRNA表达水平与Bcl-2、MMP2、MMP9、N-cadherin表达水平呈正相关(P<0.05),与Bax、E-cadherin表达水平呈负相关(P<0.05)。结论子宫肌瘤组织中miR-23呈低表达、HIF-1α呈高表达,并与ER、PR表达有关,同时可促进细胞增殖、侵袭及EMT。Objective To investigate the expression levels of microRNA-23(miR-23)and hypoxia-inducible factor-1α(HIF-1α)in uterine fibroids tissue and their correlation with cell invasion and epithelial mesenchymal transformation(EMT).Methods A total of 84 patients with uterine fibroids who underwent surgical resection in the hospital from January 2021 to January 2022 were selected as the study subjects.The uterine fibroids tissue and normal myometrium tissue(more than 0.5 cm away from the uterine fibroids)were collected for study.The expression levels of miR-23 and HIF-1αmRNA,the positive rates of estrogen receptor(ER)and progesterone receptor(PR),and the expression levels of proliferative gene[B lymphoblastoma-2(Bcl-2)],apoptotic gene[Bcl-2 associated X protein(Bax)],invasion gene[matrix metalloproteinase(MMP)2,MMP9],EMT marker gene(N-cadherin,E-cadherin)were compared between uterine fibroids tissue and normal myometrium tissue.The correlation between the expression levels of miR-23 and HIF-1αmRNA in uterine fibroids tissue and the expression levels of proliferating gene,apoptotic gene,invasion gene and EMT markers gene was analyzed.Results The expression levels of miR-23,Bax and E-cadherin in uterine fibroids tissue were lower than those in normal myometrium tissue,while the expression levels of HIF-1αmRNA,Bcl-2,MMP2,MMP9 and N-cadherin in uterine fibroids tissue were higher than those in normal myometrium tissue,the positive rates of ER and PR were higher than those in normal myometrium tissue,the differences were statistically significant(P<0.05).The expression level of miR-23 in ER and PR positive uterine fibroids tissue was lower than that in ER and PR negative uterine fibroids tissue,the expression level of HIF-1αmRNA was significantly higher than that in ER and PR negative uterine fibroids tissue,the differences were statistically significant(P<0.05).The expression level of miR-23 in uterine fibroids tissue was negatively correlated with the expression levels of Bcl-2,MMP2,MMP9 and N-cadherin(P<0.05),and posit

关 键 词：子宫肌瘤 微小RNA-23 低氧诱导因子-1Α 细胞侵袭 上皮间质转化 

分 类 号：R737.33[医药卫生—肿瘤]