hMAM、SBEM和CEACAM19 mRNA联合检测对乳腺癌的临床诊断价值  

Clinical diagnostic value of hMAM,SBEM and CEACAM19 mRNA combined detection in breast cancer

作　　者：张旭明 尹伟明 高婧[1] ZHANG Xuming;YIN Weiming;GAO Jing(Department of Clinical Laboratory,Shiyan People′s Hospital of Bao′an District,Shenzhen,Guangdong 518000,China)

机构地区：[1]深圳市宝安区石岩人民医院检验科,广东深圳518000

出　　处：《检验医学与临床》2023年第5期660-663,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨人乳腺珠蛋白(hMAM)、乳腺上皮小黏蛋白(SBEM)和癌胚抗原相关细胞黏附分子19(CEACAM19)mRNA联合检测对乳腺癌的临床诊断价值。方法选择该院2019年1月至2021年1月收治的73例乳腺癌患者作为乳腺癌组,另选取同期70例乳腺良性病变患者作为良性病变组,同期70例健康志愿者作为健康对照组。检测并比较3组hMAM、SBEM和CEACAM19 mRNA表达水平;分析乳腺癌患者临床分期及淋巴结转移情况与hMAM、SBEM和CEACAM19 mRNA表达水平的关系;通过受试者工作特征(ROC)曲线分析hMAM、SBEM和CEACAM19 mRNA单独及联合检测对乳腺癌的诊断效能。结果乳腺癌组hMAM、SBEM和CEACAM19 mRNA表达水平均高于良性病变组及健康对照组,且良性病变组上述指标表达水平均高于健康对照组,差异有统计学意义(P<0.05)。Ⅲ~Ⅳ期乳腺癌患者hMAM、SBEM和CEACAM19 mRNA表达水平均高于Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。有淋巴结转移的乳腺癌患者hMAM、SBEM和CEACAM19 mRNA表达水平均高于无淋巴结转移的患者,差异有统计学意义(P<0.05)。ROC曲线分析显示,hMAM、SBEM和CEACAM19 mRNA联合检测诊断乳腺癌的灵敏度为89.52%,特异度为65.12%,曲线下面积为0.874,高于各项指标单独检测(P<0.05)。结论hMAM、SBEM和CEACAM19 mRNA联合检测诊断乳腺癌的效能较佳,且其表达水平与临床分期及淋巴结转移有关。Objective To investigate the clinical diagnostic value of human mammary globin(hMAM),small breast epithelial mucin(SBEM)and carcinoembryonic antigen-related cell adhesion molecule 19(CEACAM19)mRNA combined detection in breast cancer.Methods A total of 73 patients with breast cancer admitted to the hospital from January 2019 to January 2021 were selected as the breast cancer group,another 70 patients with benign breast lesions during the same period were selected as the benign lesion group and 70 healthy volunteers during the same period were selected as the healthy control group.The mRNA expression levels of hMAM,SBEM and CEACAM19 in the 3 groups were detected and compared.The relationship between clinical stage,lymph node metastasis and hMAM,SBEM,CEACAM19 mRNA expression levels in patients with breast cancer were analyzed.The diagnostic efficacy of hMAM,SBEM and CEACAM19 mRNA alone and in combination was analyzed by receiver operating characteristic(ROC)curve.Results The mRNA expression levels of hMAM,SBEM and CEACAM19 in breast cancer group were higher than those in benign lesion group and healthy control group,and the above indicators in benign lesion group were higher than those in healthy control group,the differences were statistically significant(P<0.05).The mRNA expression levels of hMAM,SBEM and CEACAM19 in stageⅢtoⅣbreast cancer patients were higher than those in stageⅠtoⅡpatients,and the differences were statistically significant(P<0.05).The mRNA expression levels of hMAM,SBEM and CEACAM19 in breast cancer patients with lymph node metastasis were higher than those in patients without lymph node metastasis,and the differences were statistically significant(P<0.05).ROC curve analysis showed that the sensitivity and specificity of combined detection of hMAM,SBEM and CEACAM19 mRNA in the diagnosis of breast cancer were 89.52%,65.12%,and the area under the curve was 0.874,which was higher than that of single detection(P<0.05).Conclusion The combined detection of hMAM,SBEM and CEACAM19 mRNA is effect

关 键 词：乳腺癌 人乳腺珠蛋白 乳腺上皮小黏蛋白 癌胚抗原相关细胞黏附分子19 

分 类 号：R737.9[医药卫生—肿瘤]