PPARγ2和GPx-1基因多态性与非酒精性脂肪肝合并幽门螺杆菌感染的关联  

Association between Helicobacter pylori infection in patients with non-alcoholic fatty liver and PPARγ2 and GPx-1 gene polymorphisms

作　　者：张海娟[1] 董晓明 宋维芳[1] 师婷[1] 王美娇 郝青青[1] ZHANG Hai-juan;DONG Xiao-ming;SONG Wei-fang;SHI Ting;WANG Mei-jiao;HAO Qing-qi(Fenyang College of Shanri Medical University,Fenyang,Shanri 032200,China)

机构地区：[1]山西医科大学汾阳学院病理生理学教研室,山西汾阳032200 [2]山西省汾阳医院感染性疾病科,山西汾阳032200

出　　处：《中华医院感染学杂志》2023年第1期22-26,共5页Chinese Journal of Nosocomiology

基　　金：山西省教育厅2019年度省级大学生创新创业训练计划项目(2019815)。

摘　　要：目的 探究非酒精性脂肪肝(NAFL)合并幽门螺杆菌(Hp)感染患者过氧化物酶体增殖物激活受体γ2(PPARγ2)基因Pro12Ala位点和谷胱甘肽过氧化物酶-1(GPx-1)基因Pro198Leu位点的基因多态性。方法 选取2018年6月-2021年6月山西省汾阳医院收治的NAFL患者87例,根据是否合并Hp感染分为感染组45例和非感染组42例,分析两组患者一般资料、糖脂代谢[空腹血糖、空腹胰岛素(FINS)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、肝功能[谷丙转氨酶(ALT)、天冬氨酸转氨酶(AST)]等实验室指标,采用限制性片段长度多态性聚合酶链反应检测PPARγ2基因Pro12Ala位点、GPx-1基因Pro198Leu位点的基因分布。结果 感染组患者FINS(8.65±2.14)mIU/L、TG(1.97±0.45)mmol/L、LDL-C(3.35±1.02)mmol/L、ALT(36.38±13.04)U/L、AST(43.63±12.81)U/L水平均高于非感染组(P<0.05);两组PPARγ2基因Pro12Ala位点PP、PA、AA基因型频率和等位基因P、A频率及GPx-1基因Pro198Leu位点PP、PL、LL基因型频率和等位基因P、L频率比较差异均有统计学意义(P<0.05)。结论 PPARγ2基因Pro12Ala、GPx-1基因Pro198Leu多态性与NAFL患者合并Hp感染有关,Hp感染会导致胰岛细胞受损,影响机体血脂代谢。OBJECTIVE To explore the gene polymorphisms at Pro12Ala locus of peroxisome proliferator-activated receptor γ2(PPARγ2) gene and Pro198Leu locus of glutathione peroxidase-1(GPx-1) gene of the non-alcoholic fatty liver(NAFL) patients complicated with Helicobacter pylori(Hp) infection. METHODS A total of 87 patients with NAFL who were treated in Fenyang Hospital, Shanxi Province from Jun 2018 to Jun 2021 were enrolled in the study and divided into the infection group with 45 cases and the non-infection group 42 cases according the status of Hp infection. The baseline data and the laboratory test indexes including glucolipid metabolism indexes [fasting blood glucose, fasting insulin(FINS), triglyceride(TG), total cholesterol(TC), high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C)] and liver function indexes [alanine aminotransferase(ALT) and aspartate aminotransferase(AST)] were observed and compared between the two groups of patients. The genotypes and alleles at Pro12Ala locus of PPARγ2 gene and Pro198Leu locus of GPx-1 gene were detected by means of polymerase-chain-reaction-restriction-fragment-length polymorphism(PCR-RFLP) assay. RESULTS The levels of FINS, TG, LDL-C, ALT and AST of the infection group were respectively(8.65±2.14)mIU/L,(1.97±0.45)mmol/L,(3.35±1.02)mmol/L,(36.38±13.04)U/L and(43.63±12.81)U/L, higher than those of the non-infection group(P<0.05). There were significant differences in the frequencies of PP, PA and AA genotypes and P and A alleles at Pro12Ala locus of PPARγ2 gene and PP, PL and LL genotypes and P and L alleles at Pro198Leu locus of GPx-1 gene between the infection group and the non-infection group(P<0.05). CONCLUSION The polymorphisms at Pro12Ala locus of PPARγ2 gene and Pro198Leu locus of GPx-1 gene may be associated with the Hp infection in the NAFL patients, and the Hp infection may result in the damage of islet cells and affect the blood lipid metabolism.

关 键 词：非酒精性脂肪肝 非酒精脂肪肝病 幽门螺杆菌 过氧化物酶体增殖物激活受体γ2 谷胱甘肽过氧化物酶-1 基因多态性 

分 类 号：R575.5[医药卫生—消化系统]