Hydrogen gas and preservation of intestinal stem cells in mesenteric ischemia and reperfusion  

作　　者：Ryo Yamamoto Sayuri Suzuki Koichiro Homma Shintaro Yamaguchi Tomohisa Sujino Junichi Sasaki 

机构地区：[1]Department of Emergency and Critical Care Medicine,Keio University School of Medicine,Tokyo 1608582,Japan [2]Division of Endocrinology,Metabolism and Nephrology,Department of Internal Medicine,Keio University School of Medicine,Tokyo 1608582,Japan [3]Division of Gastroenterology and Hepatology,Department of Internal Medicine,Keio University School of Medicine,Tokyo 1608582,Japan

出　　处：《World Journal of Gastrointestinal Surgery》2022年第12期1329-1339,共11页世界胃肠外科杂志（英文版）（电子版）

摘　　要：BACKGROUND Patients with mesenteric ischemia frequently suffer from bowel necrosis even after revascularization.Hydrogen gas has showed promising effects for ischemiareperfusion injury by reducing reactive oxygen species in various animal and clinical studies.We examined intestinal tissue injury by ischemia and reperfusion under continuous initiation of 3%hydrogen gas.AIM To clarify the treatment effects and target cells of hydrogen gas for mesenteric ischemia.METHODS Three rat groups underwent 60-min mesenteric artery occlusion(ischemia),60-min reperfusion following 60-min occlusion(reperfusion),or ischemiareperfusion with the same duration under continuous 3%hydrogen gas inhalation(hydrogen).The distal ileum was harvested.Immunofluorescence staining with caspase-3 and leucine-rich repeat-containing G-protein-coupled 5(LGR5),a specific marker of intestinal stem cell,was conducted to evaluate the injury location and cell types protected by hydrogen.mRNA expressions of LGR5,olfactomedin 4(OLFM4),hairy and enhancer of split 1,Jagged 2,and Neurogenic locus notch homolog protein 1 were measured by quantitative polymerase chain reaction.Tissue oxidative stress was analyzed with immunostaining for 8-hydroxy-2'-deoxyguanosine(8-OHdG).Systemic oxidative stress was evaluated by plasma 8-OHdG.RESULTS Ischemia damaged the epithelial layer at the tip of the villi,whereas reperfusion induced extensive apoptosis of the cells at the crypt base,which were identified as intestinal stem cells with double immunofluorescence stain.Hydrogen mitigated such apoptosis at the crypt base,and the LGR5 expression of the tissues was higher in the hydrogen group than in the reperfusion group.OLFM4 was also relatively higher in the hydrogen group,whereas other measured RNAs were comparable between the groups.8-OHdG concentration was high in the reperfusion group,which was reduced by hydrogen,particularly at the crypt base.Serum 8-OHdG concentrations were relatively higher in both reperfusion and hydrogen groups without significance.CONCLUSION

关 键 词：Hydrogen molecule Intestinal ischemia Ischemia-reperfusion injury Tissue protection Nonoperative management Leucine-rich repeat-containing G-protein-coupled 5 

分 类 号：R572.3[医药卫生—消化系统]