大鼠海马区A1/A2型星形胶质细胞在弥漫性脑损伤后的表达变化  

Expression changes of A1/A2 astrocytes in hippocampus after diffuse brain injury in mice

作　　者：史文哲 宋汉君[1] 罗文哲[1] 蔡玉 盛延良[1] Shi Wenzhe;Song Hanjun;Luo Wenzhe;Cai Yu;Sheng Yanliang(Key Laboratory of Microecology-immune Regulatory Network and Related Diseases,School of Basic Medicine,Jiamusi University,Jiamusi 154000,China)

机构地区：[1]佳木斯大学基础医学院黑龙江省微生态-免疫调节网络与相关疾病重点实验室,154000

出　　处：《神经疾病与精神卫生》2022年第12期888-893,共6页Journal of Neuroscience and Mental Health

摘　　要：目的 检测大鼠在弥漫性脑损伤(DBI)后海马区A1/A2型星形胶质细胞(A1s、A2s)随时间的表达变化。方法 选取SD雄性大鼠48只,随机分为对照组和DBI后4 h、8 h、12 h、1 d、3 d、5 d、7 d组,每组6只,通过改良的Marmarou脑损伤打击装置建立大鼠脑损伤模型,通过大体观察、苏木素-伊红(HE)染色观察大鼠脑组织损伤情况,通过免疫组织化学染色、实时荧光定量聚合酶链式反应(PCR)检测各组大鼠海马区A1s、A2s标志物C3d、S100A10的蛋白及mRNA的表达变化。结果 大体观察显示,对照组大鼠未见明显异常;实验组大鼠可见局限性蛛网膜下腔出血,侧脑室和脑组织腹侧面少量出血,脑组织可见轻度水肿,未见局灶性脑挫伤及脑挫裂伤。HE染色结果显示,实验组大鼠DBI后的大脑皮层及深部脑组织形态学改变,大鼠颅脑损伤为弥漫性,DBI造模成功。免疫组织化学染色结果显示,对照组大鼠海马区C3d蛋白的平均光密度值为(0.002 6±0.000 3),DBI后4 h升高至(0.003 4±0.000 1),于DBI后1 d达高峰,为(0.015 8±0.000 4),染色程度加深,随后逐渐下降,7 d时为(0.003 6±0.000 2);DBI后8 h、12 h、1 d、3 d、5 d组大鼠的海马组织C3d蛋白的平均光密度值高于对照组,差异均有统计学意义(均P<0.05)。对照组大鼠海马区S100A10蛋白的平均光密度值为(0.078 7±0.006 8),DBI后4 h下降至(0.051 9±0.002 1),1 d时最少,为(0.005 2±0.000 2),随后上升,7 d时为(0.034 6±0.001 8);DBI后4 h、8 h、12 h、1 d、3 d、5 d、7 d组大鼠的海马组织S100A10蛋白的平均光密度值低于对照组,差异均有统计学意义(均P<0.05)。实时荧光定量PCR检测结果显示,C3d于DBI后4 h出现升高的趋势,8 h左右达高峰,表达量为(9.365 2±0.543 8)2-Δ△Ct,随后逐渐下降;S100A10在DBI 4 h后开始下降,8 h左右表达量最低,表达量为(0.446 9±0.007 8)2-Δ△Ct,随后呈上升趋势。DBI后4 h、8 h、12 h、1 d、3 d、5 d、7 d组大鼠的C3d mRNA表达量均高于�Objective To investigate the expression changes of A1/A2 astrocytes over time after diffuse brain injury (DBI) in the hippocampus of mice.Methods A total of 48 male SD mice were randomly divided into normal control group and 4 h,8 h,12 h,1 d,3 d,5 d,7 d group after DBI (n=6).The DBI model was established by modified Marmarou brain injury strike device.Brain injury in mice was observed by gross observation and hematoxylin-eosin (HE) staining.The expression of C3d,S100A10 and their mRNA,the markers of A1/A2 astrocytes (A1s/A2s) in the hippocampus of mice were detected by immunohistochemical staining and real-time quantitative Polyerase Chain Reaction (PCR).Results Gross observation showed that there was no obvious abnormality in the normal control group,but there were localized subarachnoid hemorrhage,a small amount of hemorrhage in the lateral ventricle and ventral side of brain tissue,mild edema and no focal brain contusion and laceration in the experimental group.The results of HE staining showed that the morphological changes of cerebral cortex and deep brain tissue in the experimental group after DBI,and the craniocerebral injury of mice was diffuse.DBI model was successfully established.The results of immunohistochemical staining showed that the mean density of C3d protein in the hippocampus of the normal control groupmice was(0.002 6±0.000 3).It increased to (0.003 4±0.000 1) at 4 h after DBI,and reached a peak of (0.015 8±0.000 4) at about 1 d after DBI.The staining was significantly deepened,and then the mean density gradually decreased.It was still higher than that in the normal control group at 7 d after DBI,the mean density was (0.003 6±0.000 2).The mean density of C3d protein in the hippocampus of 8 h,12 h,1 d,3 d and 5 d after DBI were all higher than that in the normal control group,and the differences were statistically significant (P<0.05).The mean density of S100A10 protein in the hippocampus of the normal control group mice was (0.078 7±0.006 8),which decreased to (0.051 9±0.002 1) at 4 h a

关 键 词：弥漫性脑损伤 星形胶质细胞 C3D S100A10 损伤时间 

分 类 号：R651.15[医药卫生—外科学]