上海市市售水产品中耐碳青霉烯类抗生素细菌分布及耐药基因谱研究  

The distribution characteristics of carbapenem-resistant bacteria in aquatic products in Shanghai markets and antibiotic resistant genes profiles

作　　者：张红芝[1] 常兆玉 於颖[1] 方沚昕 陈雯杰 陈泰尧[1] 汪静 张曦[1] ZHANG Hongzhi;CHANG Zhaoyu;YU Ying;FANG Zhixin;CHEN Wenjie;CHEN Taiyao;WANG Jing;ZHANG Xi(Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China;The Minhang District Center for Disease Control and Prevention,Shanghai 201101,China)

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]闵行区疾病预防控制中心,上海201101

出　　处：《中国食品卫生杂志》2022年第6期1128-1134,共7页Chinese Journal of Food Hygiene

基　　金：上海市自然基金(19ZR1451100);上海市公共卫生体系建设三年行动计划(GWV-2)。

摘　　要：目的了解上海市市售水产品中耐碳青霉烯类抗生素细菌的分布及抗生素耐药基因谱。方法采用美洛培南抗性平板筛选上海市市售水产品中耐药性细菌,利用微生物质谱鉴定仪对其进行鉴定,同时对上述分离细菌进行药敏性实验,对多重耐药菌进行全基因组测序,分析耐药基因谱。结果上海市市售水产品中耐碳青霉烯类抗生素的耐药细菌分离率较高(75.0%~90.5%),嗜麦芽窄食单胞菌为优势细菌,其次为假单胞菌属、气单胞菌和肠球菌;多重耐药现象比较严重,表现出对头孢类、四环素类、氨苄西林较高的耐药性。全基因组测序结果显示共检测出64个耐药基因,耐碳青霉烯酶基因以blaNDM-1、blaOXA55和blaOXA280为主。结论上海市市售水产品中耐碳青霉烯类细菌分布较为广泛,同时存在多重耐药现象,且携带的耐药基因谱呈多样性,提示应加强水产品中耐药细菌及耐药基因谱的监测,为食品安全评估提供依据。Objective To study the distribution characteristics of carbapenem-resistant bacteria in aquatic products in Shanghai markets and antibiotic resistant genes profiles.Methods Carbapenem-resistant bacteria in aquatic products were isolated using TCBS and TSA plate containing antibiotics(meropenem).The bacteria was identified using matrix assisted laser desorption/ionization time of flight mass(MALDI-TOF MS)spectrometry.The antimicrobial susceptibility was investigated using minimum inhibitory concentration(MIC).Some antibiotic resistant bacteria were analyzed using whole-genome sequencing.BioNumerics software was used to analyze and assemble whole-genome sequencing data,which was used to analyze antibiotic resistant genes profiles.Results The positive rate of carbapenem-resistant bacteria in aquatic products in Shanghai was high(75.0%~90.5%).The prevalent bacteria was Stenotrophomonas maltophilia,followed by Pseudomosa spp.,Aeromonas spp.and enterococcus spp.The multi-drug resistance was serious.The antibiotics with higher resistance rate were cephalosporins,tetracycline and ampicillin.A total of 64 antibiotic resistant genes were detected.The carbapenemase-encoding genes were blaNDM-1,blaOXA55 and blaOXA280 in this study.Conclusion The carbapenem-resistant bacteria in aquatic products in Shanghai markets was widespread.Multi-drug resistance was serious.Furthermore,the antibiotic resistant genes profiles showed diversity,which might be mechanism of occurrence of resistance.These results suggested that surveillance of resistant bacteria and antibiotic resistant genes profiles should be strengthened,which would provide scientific data for food safety risk assessment.

关 键 词：水产品 耐碳青霉烯类细菌 耐药菌 耐药基因 

分 类 号：R155[医药卫生—营养与食品卫生学]