Efficacy of luteolin on the human gastric cancer cell line MKN45 and underlying mechanism  

作　　者：DING Yajie LIU Feng LI Zhaoyan Xu Yan CAO Nida ZHANG Guangao WANG Rui ZHAO Aiguang 

机构地区：[1]Department of Oncology Medicine,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第1期34-41,共8页中医杂志（英文版）

基　　金：Supported by the National Key R&D Program(No.2017YFC1700605);the National Science and Technology Major Special Project(No.2017ZX09304001);the State Administration of Traditional Chinese Medicine,and the State Clinical Laboratory of Traditional Chinese Medicine(No.JDZX2015068)。

摘　　要：OBJECTIVE:To investigate the antitumour efficacy of luteolin on gastric cancer(GC)and study the mechanism underpinning the action.METHODS:Effects of luteolin on cell growth inhibition,apoptosis,and cell cycle arrest in MKN45 cells were investigated using the cell counting kit-8 assay.Changes in the mitochondrial membrane potential after luteolin treatment were assessed using 5,5′,6,6’-tetra-chloro-1,1’,3,3’-tetraethy lbenzimidazolcar bocyanineiodi-de(JC-1)staining.To investigate whether apoptotic effect by luteolin is related to the phosphoinositide 3-kinase/v-akt murine thymoma viral oncogene(PI3K/Akt)pathway,cells were additionally treated with LY294002,a PI3K/Akt pathway inhibitor.Moreover,the expressions of apoptosis-related proteins,namely B-cell lymphoma 2(Bcl-2),Bcl-2 associated X protein(Bax),Akt,p-Akt,caspase-3,and cytochrome C,were detected after luteolin treatment.RESULTS:The study revealed that in MKN45 cells,luteolin could inhibit the cell proliferation in a time-and dose-dependent manner;block the cell cycle in the Sphase;induce apoptosis;reduce the mitochondrial membrane potential;increase the expression of Bax,caspase-3,and cytochrome C;and decrease the expression of Bcl-2 and p-Akt.Luteolin might be involved in the PI3K/Akt signalling pathway,indicating that this pathway could be a therapeutic target for GC treatment.CONCLUSION:Luteolin could inhibit the proliferation of GC cells and block the cell cycle in the S-phase.The mechanism of inducing apoptosis in these cells was related to the PI3K/Akt signalling pathway.

关 键 词：stomach neoplasms LUTEOLIN apoptosis phosphoinositide 3-kinase Akt signal transduction 

分 类 号：R285.5[医药卫生—中药学]