Tilianin extracted from Xiangqinglan(Herba Dracocephali Moldovicae)inhibits apoptosis induced by mitochondrial pathway and endoplasmic reticulum stress in H9c2 cells after oxygen-glucose deprivation/reoxygenation  

作　　者：JIANG Wen ZHANG Wei ZHANG Yuxiang YANG Hao PAN Xiaomei CHEN Qiang CHEN Junhui 

机构地区：[1]Department of Pharmacy,the Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China [2]Department of Anesthesiology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China [3]Department of Laboratory,the Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第1期42-50,共9页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation:Mechanisms of the Protective Effects of Tilianin Against Myocardium Ischemia/Reperfusion Injury Through Ox-CaMKII-mediated Mitochondrial Regulating Pathway(No.81760045);the Xinjiang Uygur Natural Science Foundation of Autonomous Region:Study on the Mechanism of Tilianin Anti-atherosclerosis Effect of Tealis via the Reverse Cholesterol Transport Pathway(No.2018D01C317);the Chinese Medical Association Clinical Pharmacy Branch-Wu Jieping Medical Foundation:Study on the Mechanism of Tilianin Mediate Mitochondrial Function to Regulate Endoplasmic Reticulum Stress and Participate in Anti-myocardial Ischemia/Reperfusion Injury(No.320.6750.2020-04-32)。

摘　　要：OBJECTIVE:To investigate the efficacy of tilianin extracted from Xiangqinglan(Herba Dracocephali Moldovicae)on apoptosis of H9c2 cell after oxygenglucose deprivation/reoxygenation(OGD/R)and the mechanism.METHODS:Tilianin was obtained from Beijing Inluck Science and Technology Development Co.Ltd.,with purity≥98%.The OGD/R model was established in H9c2 cells.Flow cytometry detected the mitochondrial membrane potential,apoptosis rates,mitochondrial reactive oxygen species(ROS)and calcium ion concentration.Succinate dehydrogenase(SDH)activity,succinate content and levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and interleukin-1β(IL-1β)were detected with enzyme-linked immunosorbent assay.Western blot measured protein levels.RESULTS:Tilianin significantly reduced the apoptotic rates,ROS levels,calcium ion concentration,succinate content,and,levels of TNF-α,IL-6 and IL-1βof OGD/R cells,while significantly increased the membrane potential and SDH activity in mitochondria.Western blot analysis showed that tilianin significantly up-regulated pCalmodulin-dependent protein kinaseⅡand voltagedependent anion selective channel levels in OGD/R cells,while significantly down-regulated p-protein kinase B,Bcl-2-associated X,and dynamin-related protein 1 levels related to apoptosis in the mitochondrial pathway.Moreover,tilianin significantly up-regulated B-cell lymphoma-2 and mitochondrial protein 2 related to the inhibition of apoptosis.Furthermore,tilianin downregulated phosphorylated-apoptosis signal-regulated kinase 1,phosphorylated-p38 and C/EBP homologous protein related to endoplasmic reticulum stress.CONCLUSIONS:Tilianin may inhibit OGD/R-induced H9c2 cell apoptosis mediated by mitochondrial pathway and endoplasmic reticulum stress,thus protecting cardiomyocytes.

关 键 词：APOPTOSIS MITOCHONDRIA endoplasmic reticulum stress tilianin oxygen-glucose deprivation/reoxygenation 

分 类 号：R285[医药卫生—中药学]