AstragalosideⅣameliorates insulin induced insulin resistance in HepG2 cells through reactive oxygen species mediated c-Jun N-terminal kinase pathway  

作　　者：YE Xiaomei XING Xiaowei YUAN Kangrui WANG Dongming WU Dudu CHEN Zhi YU Zhiqiang 

机构地区：[1]School of Pharmacy,Guangdong Medical University,Dongguan 523808,China [2]Department of Clinical Pharmacy,SSL Central Hospital of Dongguan City(the Third People's Hospital of Dongguan City),Dongguan 523326,China [3]Department of Pharmacy,Central People's Hospital of Zhanjiang,Guangzhou 510515,China [4]School of Pharmaceutical Sciences,Guangdong Provincial Key Laboratory of New Drug Screening,Southern Medical University,Zhanjiang 524037,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第1期60-67,共8页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation of Guangdong Province(No.2017A030310666,No.2018A030307003);Guangdong Medical University Nanhai Marine Biomedical Resources R&D Public Service Platform Open Fund Project(No.2HC18013 and No.2HC18016);“Group-type”Special Support Project for Education Talents in Universities(No.4SG19045G);Undergraduate Science&Technology Innovation Foundation of Guangdong Province(No.201810571046,No.201810571073);Medical Science and Technology Development Foundation of Guangdong Province(No.A2016355);the Opening Project of State Key Laboratory of Polymer Materials Engineering(Sichuan University)(Grant No.sklpme2018-4-23)。

摘　　要：OBJECTIVE:To investigate the effects and elucidate the mechanism of Astragaloside IV(AS-IV)for insulin resistance(IR)and type 2 diabet es mellitus(T2DM).METHODS:CCK8 kit was used to detect cell viability,glucose detection kit was used to detect the concentration of glucose in cell supernatant,reactive oxygen species(ROS)detection kit and Western blot were used to explore the mechanism of Astragaloside IV(AS-IV)in improving IR.A diabetic rat model was also established by feeding high sugar and fat diet and streptozotocin(STZ)injection.After treatment with AS-IV,rosiglitazone(ROZ),or normal saline,the fasting blood glucose(FBG),C peptide(C-P),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and the glucose tolerance were assessed.RESULTS:AS-IV could effectively reduce the content of ROS and increase the glucose uptake in high insulintreated IR-type HepG 2 cells.The results of molecular mechanisms indicated that AS-IV could improve insulin resistance by reducing JNK phosphorylation and regulating c-Jun N-terminal kinase(JNK)downstream protein expression.Additionally,AS-IV could significantly reduce the levels of FBG,TNF-α,IL-6 and the glucose tolerance in diabetic rats(P<0.05 or<0.01).The high and medium dose groups of AS-IV could significantly increase the C-P levels in diabetic rats(P<0.05 or<0.01).CONCLUSIONS:Our results indicated that AS-IV improve liver IR through the JNK pathway and ROS,which meant a new molecular target for the treatment of diabetes.The AS-IV also helped to prevent and improved the insulin resistance of rats.

关 键 词：AstragalosideⅣ Insulin resistance Glucose consumption ROS JNK HepG2 cells 

分 类 号：R285[医药卫生—中药学]