Integrative phosphatidylcholine metabolism through phospholipase A_(2)in rats with chronic kidney disease  

作　　者：Yan-ni Wang Zhi-hao Zhang Hong-jiao Liu Zhi-yuan Guo Liang Zou Ya-mei Zhang Ying-yong Zhao 

机构地区：[1]School of Pharmacy,Zhejiang Chinese Medical University,No.548 Binwen Road,Hangzhou 310053,China [2]Faculty of Life Science&Medicine,Northwest University,No.229 Taibai North Road,Xi’an 710069,China [3]State Key Laboratory of Natural Medicines,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China [4]School of Food and Bioengineering,Chengdu University,Chengdu 610106,China [5]Clinical Genetics Laboratory,Affiliated Hospital&Clinical Medical College of Chengdu University,Chengdu 610081,China

出　　处：《Acta Pharmacologica Sinica》2023年第2期393-405,共13页中国药理学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.81703691,82074002);Shaanxi Key Science and Technology Plan Project(No.2019ZDLSF04-04-02);ShaanXi Science and Technology Innovation Team(No.2019TD-016).

摘　　要：Dysregulation in lipid metabolism is the leading cause of chronic kidney disease(CKD)and also the important risk factors for high morbidity and mortality.Although lipid abnormalities were identified in CKD,integral metabolic pathways for specific individual lipid species remain to be clarified.We conducted ultra-high-performance liquid chromatography-high-definition mass spectrometry-based lipidomics and identified plasma lipid species and therapeutic effects of Rheum officinale in CKD rats.Adenine-induced CKD rats were administered Rheum officinale.Urine,blood and kidney tissues were collected for analyses.We showed that exogenous adenine consumption led to declining kidney function in rats.Compared with control rats,a panel of differential plasma lipid species in CKD rats was identified in both positive and negative ion modes.Among the 50 lipid species,phosphatidylcholine(PC),lysophosphatidylcholine(LysoPC)and lysophosphatidic acid(LysoPA)accounted for the largest number of identified metabolites.We revealed that six PCs had integral metabolic pathways,in which PC was hydrolysed into LysoPC,and then converted to LysoPA,which was associated with increased cytosolic phospholipase A_(2)protein expression in CKD rats.The lower levels of six PCs and their corresponding metabolites could discriminate CKD rats from control rats.Receiver operating characteristic curves showed that each individual lipid species had high values of area under curve,sensitivity and specificity.Administration of Rheum officinale significantly improved impaired kidney function and aberrant PC metabolism in CKD rats.Taken together,this study demonstrates that CKD leads to PC metabolism disorders and that the dysregulation of PC metabolism is involved in CKD pathology.

关 键 词：chronic kidney disease LIPIDOMICS phosphatidylcholine metabolism phospholipase A_(2) Rheum officinale 

分 类 号：R69[医药卫生—泌尿科学]