迷迭香酸对宫颈癌PI3K/AKT/mTOR信号通路靶分子作用的研究  

Effects of Rosmarinic Acid on Target Molecules of PI3K/AKT/mTOR Signaling Pathway in Cervical Cancer

作　　者：覃秋莲 滕淑静[1] 胡爽 刘阳春 王志文[1,3] QIN Qiulian;TENG Shujing;HU Shuang;LIU Yangchun;WANG Zhiwen(Key Laboratory of Chronic Noncommunicable Diseases,Yueyang Vocational Technical College,Yueyang 414006,China;Nursing Department of Yueyang people’s Hospital,Yueyang 414000,China;College of Life and Health Sciences,Wuhan University of Science and Technology,Wuhan 430081,China)

机构地区：[1]岳阳职业技术学院慢性病重点实验室,岳阳414006 [2]岳阳市人民医院护理部,岳阳414000 [3]武汉科技大学生命与健康科学学院,武汉430081

出　　处：《激光生物学报》2023年第1期62-68,共7页Acta Laser Biology Sinica

基　　金：湖南省自然科学基金科教联合项目(2020JJ7071);湖南省教育厅自然科学项目(18C1770)。

摘　　要：迷迭香酸作为一种天然酚酸类化合物目前已被广泛应用于多种癌症的治疗。为探讨迷迭香酸对人宫颈癌的治疗效果,本研究通过细胞增殖、迁移和自噬等试验进行功能验证,并应用蛋白质免疫印迹技术验证了其可能发挥作用的信号通路。研究结果发现:经迷迭香酸处理后,人宫颈癌Hela细胞的增殖能力显著性下降;细胞的迁移能力同样受到抑制,自噬相关蛋白(ATGs)ATG5和Beclin1(酵母ATG6同源物)表达上调,磷脂酰肌醇-3-激酶(PI3K)、丝氨酸苏氨酸蛋白激酶(AKT)和雷帕霉素靶蛋白(m TOR)表达下降。这提示,迷迭香酸可能通过抑制PI3K/AKT/mTOR信号通路来促进细胞的自噬,并抑制宫颈癌细胞的增殖、迁移。该研究结果进一步说明了迷迭香酸作为抗癌药物的潜在价值,为抗癌药物的研发提供了一个新的理论。Rosmarinic acid, as a natural acid-secreting compound, has been widely used in the treatment of various cancers. In order to explore the therapeutic effects of rosmarinic acid on human cervical cancer. The experiments of cell proliferation, migration and autophagy were carried out in this study for functional verification, and Western blotting was used to verify the possible signaling pathways. The results showed that the proliferation ability of human cervical cancer significantly decreased after rosmarinic acid treatment;the cell migration ability was also inhibited, the expressions of autophagy-related proteins ATG5 and Beclin1 were upregulated, and the expressions of PI3K, AKT and mTOR proteins decreased. This suggests that rosmarinic acid may promote cell autophagy and inhibit the proliferation and migration of cervical cancer cells by inhibiting the PI3K/AKT/m TOR signaling pathway,thereby achieving the effects of inhibiting or treating human cervical cancer. This further indicates the potential value of rosmarinic acid as an anticancer drug. It provides a new theory for the research and development of anticancer drugs.

关 键 词：迷迭香酸 宫颈癌 细胞自噬 细胞增殖 

分 类 号：Q279[生物学—细胞生物学] R737.33[医药卫生—肿瘤]