顶空-气质联用法比较不同品种橘红胎挥发性成分差异  

Comparison of Volatile Components in Different Varieties of Citri Grandis Tomentosa Immaturus by Headspace GC-MS

作　　者：莫穗芬 方媛 杨惠琳 倪晨 詹若挺[1,2,3] 严萍 MO Suifen;FANG Yuan;YANG Huilin;NI Chen;ZHAN Ruoting;YAN Ping(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China;Chinese Medicine Resources Science and Engineering Research Center,Key Laboratory of Chinese Medicinal Resource from Lingnan(Guangzhou University of Chinese Medicine),South China Pharmaceutical Research and Development Laboratory,National Engineering and Technology Research Center of Chinese Patent Medicine,Guangzhou 510006 Guangdong,China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Maoming 525099 Guangdong,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学中药资源科学与工程研究中心、岭南中药资源教育部重点实验室(广州中医药大学),国家中成药工程技术研究中心南药研发实验室,广东广州510006 [3]岭南现代农业科学与技术广东省实验室茂名分中心,广东茂名525099

出　　处：《中药新药与临床药理》2023年第2期241-248,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：岭南现代农业科学与技术广东省实验室茂名分中心科技项目-化橘红等南药生产加工技术研究示范(2021ZZ004);广东省农村振兴战略专项—广东现代南药产业技术体系创新团队(2022KJ148);广州中医药大学2021年国家级大学生创新创业训练计划项目(202110572016)。

摘　　要：目的 建立快速分析橘红胎中挥发性成分的方法,比较不同品种橘红胎挥发性成分的差异。方法 采用静态顶空-气质联用(HS-GC-MS)技术分析橘红胎样品。采用质谱标准库(NIST17、WILEY275)检索,确定不同品种橘红胎中挥发性成分并比较差异,通过主成分分析、聚类分析对不同品种橘红胎进行归类。结果 共鉴定出橘红胎中49个挥发性成分,包括有烯类、醛类、醇类等,其中以烯类成分含量最高,超过总挥发性成分50%。副毛橘红胎中含有较高的乙酸龙脑酯,此成分在以柚类作为砧木的密叶红肉正毛、密叶黄龙正毛橘红胎中亦有检出,但含量较低。主成分分析和聚类分析均可将橘红胎按品种区分,两者的结果相一致。结论 不同品种的橘红胎挥发性成分有较大差异。所建立的HS-GC-MS分析方法稳定可靠,可用于橘红胎中挥发性成分的测定。Objective To establish a method for rapid analysis of volatile components in Citri grandis tomentosa immaturus,and to compare the differences of volatile components in different varieties of Citri grandis tomentosa immaturus. Methods Static headspace gas chromatography mass spectrometry(HS-GC-MS) was used to analyze the samples of Citri grandis tomentosa immaturus. Volatile components in different varieties of Citri grandis tomentosa immaturus were identified by comparison with the standard mass spectra library(NIST17,WILEY275).Then different varieties of Citri grandis tomentosa immaturus were classified by principal component analysis and cluster analysis. Results A total of 49 volatile components were identified, including alkenes, aldehydes,alcohols,etc. Among them,the content of alkenes was the highest,more than 50% of the total volatile components.A higher content of bornyl acetate was found in Citri grandis tomentosa immaturus with less fluff. This component was also detected in two other varieties,such as Miye Hongrou Citri grandis tomentosa and Miye Huanglong Citri grandis tomentosa, which can be used as rootstock. Both principal component analysis and cluster analysis can distinguish varieties of Citri grandis tomentosa immaturus, and the results are consistent. Conclusion There are obvious differences in volatile components among different varieties of Citri grandis tomentosa immaturus. The established HS-GC-MS method is stable and reliable. It can be used for the determination of volatile components in Citri grandis tomentosa immaturus.

关 键 词：橘红胎 挥发性成分 顶空-气相色谱-质谱联用 主成分分析 聚类分析 品种差异 

分 类 号：R284.1[医药卫生—中药学]