不同培养和储藏条件对北美豆梨、我国杜梨花粉萌发的影响  

Effects of Different Culture and Storage Conditions on Pollen Germination of Pyrus calleryana and Pyrus betulaefolia

作　　者：邓伟 王一菲 李泽根 路丙社[1] 路斌[1] DENG Wei;WANG Yifei;LI Zegen;LU Bingshe;LU Bin(College of Landscape Architecture and Tourism,Hebei Agricultural University,Baoding Hebei,071000,China)

机构地区：[1]河北农业大学园林与旅游学院,河北保定071000

出　　处：《河北科技师范学院学报》2022年第4期13-18,共6页Journal of Hebei Normal University of Science & Technology

基　　金：河北省重点研发计划项目(项目编号:20326329D);河北省自然科学基金项目(项目编号:C2021204126)。

摘　　要：为探明北美豆梨和杜梨花粉萌发的适宜条件和最佳液体培养基组分,以北美豆梨‘克利夫兰’‘三体’和我国的杜梨花粉为材料进行了培养温度,蔗糖、H_(3)BO_(3)和Ca(NO_(3))_(2)质量浓度以及贮藏方式对花粉萌发影响的试验,利用L_(16)(4^(3))正交试验设计对液体培养基组分进行了筛选优化。结果表明:杜梨及北美豆梨花粉萌发的适宜温度为25℃,蔗糖质量浓度为150 g·L^(-1);适宜杜梨、‘克利夫兰’和‘三体’花粉萌发的H3BO3质量浓度分别为50,100,50 mg·L^(-1),Ca(NO_(3))_(2)质量浓度分别为50,100,50 mg·L^(-1);-20℃和-40℃冷冻贮藏是3种花粉保存的最佳方式;杜梨、‘克利夫兰’和‘三体’花粉萌发的最适液体培养基分别为:150 g·L^(-1)蔗糖+100 mg·L^(-1)Ca(NO_(3))_(2)+50 mg·L^(-1)H_(3)BO_(3),100 g·L^(-1)蔗糖+100 mg·L^(-1)Ca(NO_(3))_(2)+150 mg·L^(-1)H_(3)BO_(3),130 g·L^(-1)蔗糖+50 mg·L^(-1)Ca(NO_(3))_(2)+150 mg·L^(-1)H3BO3。The effects of culture temperature,sucrose,boric acid and calcium nitrate concentrations and storage methods on pollen germination were studied with Pyrus calleryana and Pyrus betulaefolia pollen as materials to find out the suitable conditions and the best liquid medium components for pollen germination of Pyrus calleryana and Pyrus betulaefolia.The liquid medium components were screened and optimized by L_(16)(4^(3))orthogonal experiments.The results showed that the suitable temperature for pollen germination of Pyrus betulaefolia and Pyrus calleryana was 25℃,and the sucrose concentration was 150 g·L^(-1).The suitable boric acid concentration for pollen germination of Pyrus betulaefolia,‘Cleveland’and‘Trisomy’were 50,100,50 mg·L^(-1),and the suitable calcium nitrate concentration were 50,100,50 mg·L^(-1).-20℃and-40℃frozen storage is the best way to preserve three kinds of pollen;the optimum liquid medium for pollen germination of Pyrus betulaefolia,‘Cleveland’and‘Trisomic’were:150 g·L^(-1)sucrose+100 mg·L^(-1)calcium nitrate+50 mg·L^(-1)boric acid,100 g·L^(-1)sucrose+100 mg·L^(-1)calcium nitrate+150 mg·L^(-1)boric acid,130 g·L^(-1)sucrose+50 mg·L^(-1)calcium nitrate+150 mg·L^(-1)boric acid,respectively.

关 键 词：杜梨 北美豆梨 花粉 贮藏方式 萌发率 

分 类 号：S685.99[农业科学—观赏园艺]