FAAS法测定海带中微量元素的不确定度评定  

Uncertainty Evaluation of Trace Elements Content in Kelp(Laminaria japonica) by FAAS

作　　者：刘兰英 吕新 李莹 罗土炎 上官亮 李玥仁 LIU Lanying;LÜXin;LI Ying;LUO Tuyan;SHANGGUAN Liang;LI Yueren(Institute of Agricultural Quality Standards and Testing Technology Research,Fujian Academy of Agricultural Science,Fuzhou 350003;Fujian Key Laboratory of Agro-products Quality&Safety,Fuzhou 350003)

机构地区：[1]福建省农业科学院农业质量标准与检测技术研究所,福州350003 [2]福建省农产品质量安全重点实验室,福州350003

出　　处：《食品工业》2023年第1期320-324,共5页The Food Industry

基　　金：福建省公益类科研院所专项(2020R1022002);福建省农业科学院科技创新团队建设项目(CXTD2021011-1);福建省“5511”协同创新工程项目(XTCXGC2021020)。

摘　　要：为提高实验室火焰原子吸收光谱法测定海带中微量元素含量的准确性,采用火焰原子吸收光谱法(FAAS)测定海带样品中铁、锌含量,结合试验过程和数学模型,分析不确定度来源,量化每个潜在来源所产生的不确定度,并将其合成,得到合成标准不确定度和扩展不确定度。结果表明:海带样品中铁含量252 mg/kg时,其扩展不确定度为14 mg/kg (k=2);锌含量15.9 mg/kg时,其扩展不确定度为2.6 mg/kg (k=2)。对海带中铁、锌测量结果影响最大的不确定度来源是样品溶液质量浓度,其次是方法回收率和测量重复性。可通过优化标准溶液配制过程、调整校准曲线浓度范围、优化样品前处理方法等措施降低不确定度,确保检测结果的准确性。To improve the accuracy of the results of Fe and Zn contents in kelp by flame atomic absorption spectrometry(FAAS), the concentrations of Fe and Zn elements in kelp sample were measured by using FAAS. Combining with the experimental process and mathematical model, the sources of measurement uncertainty were firstly analyzed, then the uncertainties generated by each potential source were quantified and synthesized, and the combined standard uncertainty and expanded uncertainty were obtained. The results showed that when the value of Fe concentration in kelp was 252 mg/kg, the expanded uncertainty was 14 mg/kg(k=2);The value of Zn concentration was 15.9 mg/kg, the expanded uncertainty was 2.6mg/kg(k=2). The uncertainty introduced by the elements concentration of sample solution has the greatest influence on the measurement results of Fe and Zn in kelp, followed by the method recovery rate and measurement repeatability. In order to ensure the accuracy of the result, the measurement uncertainty could be reduced by optimizing standard solution preparation process, selecting suitable calibration curve range, and optimizing sample pretreatment method.

关 键 词：火焰原子吸收光谱法(FAAS) 微量元素 不确定度 海带 标准溶液 

分 类 号：O657.31[理学—分析化学] TS254.58[理学—化学]