MUC1模拟表位肽致敏DC治疗宫颈癌荷瘤小鼠  

MUC1 mimotope peptide sensitized DC in the treatment of cervical cancer-bearing mice

作　　者：徐建勤[1] 袁军[1] 吴超琦 周炜根 XU Jianqin;YUAN Jun;WU Chaoqi;ZHOU Weigen(Jiaxing Maternity and Child Health Care Hospital,Jiaxing,Zhejiang 314001,China)

机构地区：[1]浙江省嘉兴市妇幼保健院,浙江嘉兴314001

出　　处：《中国优生与遗传杂志》2023年第2期236-242,共7页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究黏蛋白1(MUC1)模拟表位肽致敏树突状细胞(DC)对宫颈癌荷瘤小鼠生长和T淋巴细胞亚群的影响。方法培养小鼠骨髓细胞,诱导并刺激DC成熟,将MUC1模拟表位肽加入成熟DC,形成负载MUC1模拟表位肽的DC。建立稳定表达MUC1的宫颈癌U14细胞株荷瘤BALB/c小鼠模型。空白对照组注射0.2 mL生理盐水;DC组注射2×10^(5)个未加MUC1模拟表位肽的DC;DC+MUC1组注射2×10^(5)个经过负载MUC1模拟表位肽的DC。免疫1周后,进行二次免疫。结果肿瘤体积与治疗前比较,空白对照组、DC组和DC^(+)MUC1组均升高(P<0.05);治疗前,各组间比较差异无统计学意义(P>0.05);二次免疫1周后,DC^(+)MUC1组低于空白对照组和DC组(P<0.05),空白对照组和DC组间差异无统计学意义(P>0.05);小鼠抑瘤率DC组和DC+MUC1组均高于空白对照组(P<0.05),DC+MUC1组高于DC组(P<0.05);小鼠外周血CD3^(+)、CD4^(+)、CD8^(+)水平DC^(+)MUC1组高于空白对照组和DC组(P<0.05),空白对照组和DC组间差异无统计学意义(P>0.05);CD4^(+)CD25^(+)FOXP3^(+)Treg水平和小鼠血清白介素6(IL-6)、白介素10(IL-10)、转化生长因子β(TGF-β)、血管内皮因子(VEGF)水平DC+MUC1组低于空白对照组和DC组,空白对照组和DC组间差异无统计学意义(P>0.05)。结论MUC1模拟表位肽致敏DC可抑制宫颈癌荷瘤小鼠肿瘤生长,调节T淋巴细胞亚群水平,改善细胞因子表达。Objective To study the effect of MUC1 mimotope peptide-sensitized dendritic cells(DC)on the growth and T lymphocyte subsets of cervical cancer-bearing mice.Methods The mouse bone marrow cells are cultured to induce and stimulate DC maturation,and MUC1 mimotope peptides are added to mature DC to form DCs loaded with MUC1 mimotope peptides.Establish a cervical cancer U14 cell line cancer-bearing BALB/c mouse model.The blank control group was injected with 0.2 mL of saline,the DC group was injected with 2×10^(5) DCs without MUC1 mimotope peptides,the DC+MUC1 group was injected with 2×10^(5) DCs with MUC1 mimotope peptides loaded.After 1 week of immunization,a second immunization was performed.Results Compared with the tumor volume before treatment,the blank control group,DC group and DC+MUC1 group all increased(P<0.05).Before treatment,there was no statistically significant difference between the groups(P>0.05).1 week after the second immunization,the DC+MUC1 group was lower than the blank control group and the DC group(P<0.05),and there was no statistically significant difference between the blank control group and the DC group(P>0.05).The tumor suppression rate of mice in DC group and DC+MUC1 group were higher than in blank control group(P<0.05),DC+MUC1 group was higher than DC group(P<0.05).The peripheral blood CD3^(+),CD4^(+),CD8^(+)levels in the DC+MUC1 group were higher than those in the blank control group and the DC group(P<0.05),and there was no statistically significant difference between the blank control group and the DC group(P>0.05).CD4^(+)CD25^(+)FOXP3^(+)Treg levels and mouse serum interleukin 6(IL-6),interleukin 10(IL-10),transforming growth factorβ(TGF-β)and vascular endothelial factor(VEGF)levels in DC+MUC1 group were all lower than those in blank control group and DC group.There was no statistical difference between blank control group and DC group significance(P>0.05).Conclusion MUC1 mimotope peptide-sensitized DC can inhibit tumor growth in cervical cancer-bearing mice,regulate T lymphocyte

关 键 词：黏蛋白1 模拟表位肽 树突状细胞 宫颈癌 T淋巴细胞亚群 

分 类 号：R737.33[医药卫生—肿瘤]