AMPK/NF-κB参与牙龈卟啉单胞菌脂多糖诱导的人牙周韧带细胞的炎症反应  

AMPK/NF-κB pathway participate in Porphyromonas gingivalis lipopolysaccharide-induced inflammatory responses in hPDLCs

作　　者：李归平 成益凡 古丽其合热·阿布来提 秦旭 朱光勋[1] LI Gui-ping;CHENG Yi-fan;Guliqihere·Abulaiti;QIN Xu;ZHU Guang-xun(Department of Stomalogy,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院口腔科,湖北武汉430030

出　　处：《临床口腔医学杂志》2023年第1期7-11,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金(编号81300883、81800981);湖北省自然科学基金(编号2019CFB688、2021CFB056)。

摘　　要：目的:探索牙龈卟啉单胞菌脂多糖(Porphyromonas gingivalis lipopolysaccharide, PgLPS)刺激对人牙周韧带细胞(human periodontal ligament cells, hPDLCs)引起炎症反应的机制的影响。方法:体外培养hPDLCs,以PgLPS梯度浓度进行干预,CCK-8法检测细胞活性,qRT-PCR法检测细胞IL-6、IL-8、AMPK、NF-κB mRNA的表达水平,ELISA法检测上清IL-6、IL-8的浓度,Western Blot法检测细胞腺苷酸活化蛋白激酶(AMP-activated protein kinase, AMPK)、核转录因子κB(nuclear factor-kappa B,NF-κB)、核转录因子抑制蛋白α(inhibitor of NF-κBα,IκBα)的蛋白活性。结果:与对照组相比,PgLPS刺激能提高细胞IL-6、IL-8分泌,降低细胞p-AMPK表达,增强p-IκBα、p-NF-κB表达,且均呈现浓度依赖性(P<0.05);AMPK、NF-κB、IL-6和IL-8的核酸表达水平(mRNA)与蛋白变化一致(P<0.05)。结论:在体外培养的hPDLCs中,AMPK/NF-κB信号通路可能参与了PgLPS诱导的炎症反应。Objective:To explore the effect of Porphyromonas gingivalis lipopolysaccharide(PgLPS)on the mechanism of inflammatory response in human periodontal ligament cells(hPDLCs).Methods:Cultured hPDLCs were stimulated with PgLPS in concentration gradient.CCK-8 assay was used to detect cellular activity.qRT-PCR was used to detect the expression levels of IL-6,IL-8,AMPK and NF-κB mRNA.ELISA was used to detect the concentrations of IL-6 and IL-8 in supernatant.Western Blot was used to detect the protein activity of AMP-activated protein kinase(AMPK),nuclear factor-kappa B(NF-κB)and inhibitor of NF-κBα(IκBα).Results:Compared with the control group, PgLPS increased cellular IL-6 and IL-8 secretion, decreased cellular p-AMPK expression and enhanced p-IκBα and p-NF-κB expression, with a concentration-dependent manner(P<0.05).RNA expression levels of AMPK,NF-κB,IL-6 and IL-8 were consistent with protein changes(P<0.05).Conclusion:In vitro cultured hPDLCs, the AMPK/NF-κB signaling pathway may involved in the PgLPS-induced inflammatory response.

关 键 词：牙龈卟啉单胞菌脂多糖 腺苷酸活化蛋白激酶 炎症 人牙周韧带细胞 

分 类 号：R781.4[医药卫生—口腔医学] Q813.1[医药卫生—临床医学]