Delivery of Micro RNAs by plant virus-based nanoparticles to functionally alter the osteogenic differentiation of human mesenchymal stem cells  

作　　者：Fei Xue Jeroen J.L.M.Cornelissen Quan Yuan Shuqin Cao 

机构地区：[1]State Key Laboratory of Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China [2]Department of Molecules&Materials,MESA+Institute for Nanotechnology,University of Twente,Enschede AE 7500,the Netherlands

出　　处：《Chinese Chemical Letters》2023年第1期215-220,共6页中国化学快报（英文版）

基　　金：supported by the research funding from West China School/Hospital of Stomatology,Sichuan University(Nos.RCDWJS2021-15,RD-03-202010,RD-02-202004);the research funding from the State Key Laboratory of Oral Diseases(No.SKLOD202111);the fellowship of China Postdoctoral Science Foundation(No.2020TQ0211)。

摘　　要：Micro RNA-26a(miR-26a)has been verified to promote osteogenic differentiation of mesenchymal stem cells in recent years.The main obstacles to its application in bone regeneration are instability in the physiological environment and low efficiency of cellular membrane penetration.To overcome these problems,we constructed a novel plant virus gene delivery system based on Cowpea chlorotic mottle virus(CCMV).By encapsulating miR-26a with purified capsid protein(CP)dimers derived from CCMV,CPmiR-26a(CP26a)virus-like particles(VLPs)were obtained.CP26a retained a structure similar to the native CCMV and protected miR-26a from digestion with its exterior CP.Moreover,CP26a featured similar cellular uptake efficiency,osteogenesis promotion ability,and better biocompatibility compared with Lipofectamine2000-miR-26a(lipo26a),which indicated a promising prospect for CCMV as a novel gene delivery system.

关 键 词：VLPS Cowpea chlorotic mottle virus Gene delivery MICRORNAS OSTEOGENESIS 

分 类 号：Q94[生物学—植物学]