河北省鼠疫自然疫源地小肠结肠炎耶尔森菌和假结核耶尔森菌的调查研究  

Investigation of Yersinia pseudotuberculosis and Y. enterocolitica in the natural focus of plague in Hebei province, China

作　　者：牛艳芬[1] 周松[1] 刘广[1] 刘晓伟 张懿晖[1] 王海峰[1] 段然[2] 王鑫[2] 杨晓燕[1] 杜越聪 王汐 赵升 金春霞 杜国义[1] 史献明[1] 崔耀仁[1] 闫萍 NIU Yan-fen;ZHOU Song;LIU Guang;LIU Xiao-wei;ZHANG Yi-hui;WANG Hai-feng;DUAN Ran;WANG Xin;YANG Xiao-yan;DU Yue-cong;WANG Xi;ZHAO Sheng;JIN Chun-xia;DU Guo-yi;SHI Xian-ming;CUI Yao-ren;YAN Ping(Department of Laboratory/Department of Epidemiology,Anti-plague Institute of Hebei Province,Zhangjiakou,Hebei 075000,China;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Institute for Endemic Disease of Zhangjiakou,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北省鼠疫防治所检验科/流行病科,河北张家口075000 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]张家口市地方病防治所,河北张家口075000

出　　处：《中国媒介生物学及控制杂志》2023年第1期91-93,共3页Chinese Journal of Vector Biology and Control

基　　金：河北省2021年度医学科学研究课题计划(20210359);河北省2020年度医学科学研究课题计划(20200810);国家科技重大专项(2018ZX10713-001-002,2018ZX10713-003-002)。

摘　　要：目的 调查河北省鼠疫自然疫源地小肠结肠炎耶尔森菌和假结核耶尔森菌分布情况,为鼠传疾病防控提供科学依据。方法 采集宿主动物舌根部和回盲肠部标本,分别用培养、PCR方法检测小肠结肠炎耶尔森菌和假结核耶尔森菌,并采用Fisher确切概率法比较阳性率的差异。结果 共采集样本1 048份,其中舌根部543份,回盲肠部505份。致病性耶尔森菌细菌培养全部阴性。假结核耶尔森菌PCR初筛全部阴性。小肠结肠炎耶尔森菌PCR初筛铁草胺菌素受体蛋白基因(foxA)阳性2份,年阳性率为0.41%(2/490),舌根部和回盲肠部标本foxA阳性率差异无统计学意义(χ^(2)=4.629,P=0.112)。结论 河北省鼠疫自然疫源地部分地区主要宿主携带小肠结肠炎耶尔森菌,鼠疫监测同时需要加强其他致病性耶尔森菌的监测。Objective To investigate the distribution of Yersinia pseudotuberculosis and Y. enterocolitica in the natural focus of plague in Hebei province, China, and to provide a scientific basis for rodent borne diseases prevention and control. Methods Samples from the lingual root and ileocecum of host animals were collected, and culture and polymerase chain reaction(PCR) were used to detect Y. enterocolitica and Y. pseudotuberculosis. Results A total of 1 048 samples were collected, including 543 samples from the lingual root and 505 samples from the ileocecum. All bacterial cultures were negative for pathogenic Yersinia. All samples were negative for Y. pseudotuberculosis with PCR screening. Two foxA+(ferrioxamine receptor gene) samples for Y. enterocolitica were screened out by PCR, and the annual positive rate was 0.41%(2/490). Conclusion The main host in some natural foci of plague in Hebei province carries Y. enterocolitica, and the surveillance of other pathogenic Yersinia should be strengthened at the same time of plague surveillance.

关 键 词：河北省鼠疫自然疫源地 小肠结肠炎耶尔森菌 假结核耶尔森菌 调查 

分 类 号：R378.6[医药卫生—病原生物学]