多指标综合评价肉苁蓉多糖的抗氧化活性  

Comprehensive evaluation of antioxidant activity of Cistanche deserticola polysaccharides by multiple indexes

作　　者：周永晟 蔡俊杰 高连春 孟欣睿 王梦含 刘小花[1] Zhou Yongsheng;Cai Junjie;Gao Lianchun;Meng Xinrui;Wang Menghan;Liu Xiaohua(School of Pharmaceutic,Lanzhou University,Lanzhou 730000,China;Guangzhou Yuming Biological Technology Co,LTD,Guangzhou 511400,China)

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]广州煜明生物科技有限公司,广东广州511400

出　　处：《甘肃医药》2022年第12期1057-1060,共4页Gansu Medical Journal

基　　金：甘肃省科学技术计划项目(编号:18CX2ND005)。

摘　　要：目的:通过体内、外抗氧化实验,多种指标综合评价肉苁蓉多糖抗氧化活性。方法:体外抗氧化实验观察肉苁蓉多糖对自由基的清除率[包括DPPH清除法、羟自由基(·OH)清除法、铁氰化钾还原法、亚硝酸盐清除法];体内抗氧化实验采用D-半乳糖小鼠衰老模型,观察小鼠的体重和脾脏指数,检测血清、肝组织、脑组织的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)的含量。结果:体外抗氧化实验中肉苁蓉多糖(C80)具有很好地清除DPPH自由基和羟自由基效果;在小鼠衰老模型中,与模型组相比较,C80可以提升小鼠增重率与脾脏指数(P<0.05),其血清、肝组织、脑组织的SOD、GSH-Px活性和MDA含量具有显著性差异(P<0.01),且高低剂量组组间无差异(P>0.05)。结论:C80在体内、体外均具有良好的抗氧化活性。Objective:To evaluate the antioxidant activity of Cistanche deserticola polysaccharides by various indexes in vivo and vitro.Methods:Antioxidant experiments in vitro focused on the free radical scavenging rate of Cistanche deserticola polysaccharides including DPPH scavenging,hydroxyl radical(·OH)scavenging,potassium ferricyanide reduction and nitrite scavenging method.The body weight and spleen index of mice were concerned,and the contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in serum,liver and brain tissues were detected in d-galactose aging model mice.Results:C80 had better DPPH and OH·scavenging effect in vitro.The weight gain rate and spleen index of mice(P<0.05)were improved,and the SOD,GSH-Px activities and MDA content in serum,liver and brain tissues had significant differences(P<0.01)using C80 could in mouse aging model,but there was no difference between high and low dose groups(P>0.05).Conclusion:C80 has good antioxidant activity in vivo and vitro.

关 键 词：肉苁蓉多糖 自由基 清除率 抗氧化活性 衰老模型 

分 类 号：R285.5[医药卫生—中药学]