胀包郫县豆瓣中耐盐鲁氏接合酵母的筛选和产气能力分析  

Screening and Gas Production Capacity Analysis of a Halotolerant Zygosaccharomyces rouxii Strain Isolated from the Swollen Packages of Pixian Broad Bean-chili Paste

作　　者：范智义 王泽亮 张其圣 陈功 邓维琴 冉亚琳 李恒 FAN Zhiyi;WANG Zeliang;ZHANG Qisheng;CHEN Gong;DENG Weiqin;RAN Yalin;LI Heng(Sichuan Academy of Food and Fermentation Industries Co.Ltd.,Chengdu 611130,China;SichuanDongpo Chinese Paocai Industrial Technology Research Institute,Meishan 620000,China)

机构地区：[1]四川省食品发酵工业研究设计院有限公司,四川成都611130 [2]四川东坡中国泡菜产业技术研究院,四川眉山620000

出　　处：《现代食品科技》2023年第2期152-160,共9页Modern Food Science and Technology

基　　金：国家重点研发计划项目(2016YFD0400505);成都市科技计划项目(2018-YF09-00036-SN)。

摘　　要：郫县豆瓣是我国西南地区的传统调味品,胀包是市售郫县豆瓣最主要的质量缺陷。该研究采集了郫县豆瓣和其两种半成品,甜瓣子和辣椒醅,测定了其食盐含量和水分活度(Water Activities,aw),并筛选了其中的耐盐微生物。结果表明,产气的样品食盐含量相对较低,a w相对较高。将样品稀释液接种于含12%(m/m)氯化钠的YM肉汤培养基中,可分离出一种耐盐微生物,鲁氏接合酵母(Zygosaccharomyces rouxii)。鲁氏接合酵母在YM肉汤培养基,或者回接入食盐含量低于16%(m/m)的灭菌甜瓣子、辣椒醅和郫县豆瓣中,均可导致产气现象发生。其最适生长条件为含盐量4%(m/m),pH值4~6及30℃。其在含盐量8%(m/m)、pH值5~7的情况下产气量最大。提高培养基或灭菌样品的盐含量,鲁氏接合酵母的产气受抑。鲁氏接合酵母可在酸性高盐条件下生长,并能够在郫县豆瓣样品中代谢产气,导致其胀包。Pixian broad bean-chili paste(PBCP)is a traditional condiment in Southwest China,and package swelling is one of the major quality flaws in commercial PBCP.In this study,PBCP and its two semi-finished products,namely broad bean moromi(BM)and pickled chili(PC)paste were collected.Salt contents and water activities(aw)of the samples were determined and the halotolerant microorganisms were isolated.The results showed that the gas-producing samples had relatively lower salt contents and higher a w values.By inoculating the diluted sample into YM broth media containing 12%(m/m)of NaCl,Zygosaccharomyces rouxii,a halotolerant yeast,was isolated.The production of gas could be induced through inoculating the yeast in YM broth medium,or by re-inoculating the yeast in the autoclaved BM,PC and PBCP with salt contents lower than 16%(m/m).The optimal growth conditions for Z.rouxii were 4%(m/m)NaCl,pH 4~6 and 30℃,with the gas production being largest at 8%(m/m)NaCl and pH 5~7.Gas production by Z.rouxii was inhibited when the salt content increased in the culture medium or the sterilized samples.Z.rouxii can grow under acidic and high-salt conditions and can produce gas in PBCP,causing package swelling.

关 键 词：质量缺陷 盐含量 产气 耐盐 控制 

分 类 号：TS264.24[轻工技术与工程—发酵工程]