盐制巴戟天多糖结构表征及其免疫活性  

Structure Characterization and Immunological Activity Evaluation of the Polysaccharides from Salt-processed Morinda officinalis

作　　者：赖岚玉 罗志锋 朱思阳 陶倩 黎攀 杜冰[1] LAI Lanyu;LUO Zhifeng;ZHU Siyang;TAO Qian;LI Pan;DU Bing(College of Food Science,South China Agricultural University,Guangzhou 510642,China;Infinitus(China)Co.Ltd.,Guangzhou 510623,China;Huaantang Biological Technology Group Co.Ltd.,Guangzhou 511500,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]无限极(中国)有限公司,广东广州510623 [3]花安堂生物科技集团有限公司,广东广州511500

出　　处：《现代食品科技》2023年第2期197-204,共8页Modern Food Science and Technology

基　　金：财政部和农业农村部国家现代农业产业技术体系资助(CARS-21);广东省自然科学基金面上项目(2020A151501268);广东省重点领域研发计划项目(2020B020226008)。

摘　　要：该研究采用水提醇沉法从盐制巴戟天中提取粗多糖(SMP),经DEAE-52纤维素柱和葡聚糖凝胶Sephadex G 100柱分离纯化得到多糖(SMP-4),采用红外光谱、高效凝胶渗透凝胶色谱、气相色谱和核磁共振波对SMP-4进行结构表征,并以RAW264.7巨噬细胞为模型,通过测定其细胞增殖活性和细胞因子分泌水平来评价其免疫活性。多糖结构分析表明,SMP-4是由阿拉伯糖、半乳糖、鼠李糖、核糖、木糖、葡萄糖和甘露糖组成的,摩尔比例为:0.55:0.23:0.16:0.03:0.02:0.01:0.01,分子量为1.32×10^(5) u的多糖。SMP-4具有六种糖苷键,其中→3)-D-Araf-(1→占比最高,为58.35%。在免疫活性评价分析中表明,与对照组相比,在15.625~500.000μg/mL质量浓度范围内不影响RAW264.7细胞的增殖,同时能显著地促进肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)的分泌。SMP-4质量浓度为500μg/mL时,RAW264.7细胞分泌的TNF-α质量浓度最高,为2100.00 pg/mL(P<0.001);在31.25μg/mL质量浓度下,RAW264.7细胞分泌的IL-1β质量浓度最高,为14.50 pg/mL(P<0.05)。综上,SMP-4表现出良好免疫活性,具有成为一种天然的免疫调节剂的潜力。In this study the polysaccharides from salt-processed Morinda officinalis SMP was extracted by water extraction and ethanol precipitation,and then purified sequentially by DEAE-52 cellulose and Sephadex-G100 columns,to yield a purified polysaccharide(named SMP-4).Fourier transform infrared spectroscopy,high performance gel permeation chromatography,gas chromatography and nuclear magnetic resonance were employed to characterize the structural properties of SMP-4,and its immunomodulatory activities were examined in Macrophage RAW264.7 through determining its cell proliferation activity and levels of secreted cytokines.The polysaccharide structure analysis showed that SMP-4 consisted of arabinose,galactose,rhamnose,ribose,xylose,glucose and mannose in the molar ratio of 0.55:0.23:0.16:0.03:0.02:0.01:0.01,and had a molecular weight of 1.32×10^(5) u.SMP-4 had six kinds of glycosidic bonds,with→3)-D-linked-Araf-(1→accounting for the highest proportion(58.35%).The immunological activity analyses showed that the proliferation of RAW264.7 cells was not affected in the concentration range of 15.625~500.000μg/mL as compared with the control group,whilst the secretions of tumour necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were significantly promoted.At a SMP-4 concentration of 500μg/mL,the amount of TNF-αsecreted by RAW264.7 cells reached the highest level(2100.00 pg/mL)(P<0.001);At a SMP-4 concentration of 31.25μg/mL,RAW264.7 cells secreted the highest amount of IL-1β(14.50 pg/mL)(P<0.05).In conclusion,SMP-4 exhibited a good immune activity and has the potential to be a natural immunomodulator.

关 键 词：盐制巴戟天 分离纯化 多糖 结构表征 免疫活性 

分 类 号：TS205[轻工技术与工程—食品科学]