荧光探针在羧酸酯酶检测中的研究进展  

Progress in Fluorescent Probes for Carboxylesterase Detection

作　　者：冯静怡 刘永国[1] 田红玉[1] 杨绍祥[1] Feng Jingyi;Liu Yongguo;Tian Hongyu;Yang Shaoxiang(School of Light Industry,Beijing Technology and Business University,Beijing,100048)

机构地区：[1]北京工商大学轻工科学技术学院,北京100048

出　　处：《化学通报》2023年第2期179-188,共10页Chemistry

基　　金：国家自然科学基金项目(31901770)资助。

摘　　要：羧酸酯酶(CES)属于丝氨酸酯酶超家族,它们位于多种组织的内质网和胞质,参与药物、环境毒物和前致癌物的代谢激活或发挥解毒作用。为进一步了解CES生理学和药理学的作用,研发出一种能够在细胞、组织和有机体水平上实时准确监测CES的方法非常有必要。荧光探针具有高选择性、高灵敏度、快速检测、比色明显、操作简便、实时成像等优势,作为一种灵敏的检测手段,被广泛应用于生物检测、光学成像等领域。本文主要对近5年来用于CES检测的荧光探针研究进展进行总结。Carboxylesterases(CES)belong to the serine esterase superfamily,which are located in the endoplasmic reticulum and cytoplasm of many tissues and are involved in the metabolic activation or detoxification of drugs,environmental toxicants and procarcinogens.To further understand the role of carboxylesterases in physiology and pharmacology,it is necessary to develop a method that can accurately monitor carboxylesterases in real time at the cellular,tissue and organismal levels.With the advantages of high selectivity,high sensitivity,rapid detection,obvious colorimetry,easy operation,and real-time imaging,fluorescent probes were widely used as a sensitive detection method in bioassays and optical imaging.This paper mainly summarized the research progress in fluorescent probes for the detection of carboxylesterases during the past 5 years.

关 键 词：羧酸酯酶 荧光探针 

分 类 号：O657.3[理学—分析化学] R313[理学—化学]