基于山苍子精油的复配筛选及协同抗菌性能  

Compound screening and synergistic antibacterial properties of litsea cubeba essential oils

作　　者：王颖 李琳 胡静 WANG Ying;LI Lin;HU Jing(School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China)

机构地区：[1]上海应用技术大学香料香精化妆品学部,上海201418

出　　处：《精细化工》2023年第1期146-152,199,共8页Fine Chemicals

基　　金：国家自然科学基金(22078196);上海市曙光计划项目(19SG52);上海市自然科学基金项目(22ZR1460400);上海应用技术大学引进人才科研启动项目(YJ2021-86)。

摘　　要：为解决单一植物精油在抗菌应用时用量相对较大、气味单一且浓郁刺激、易引起人们感官不适及过敏反应等问题。通过研究13种单一植物精油对大肠杆菌和金黄色葡萄球菌的抑菌效果,以山苍子精油为基调,采用棋盘稀释法、抑菌圈法、感官评价法筛选出具有协同抗菌效果的芳香复配精油,并以大肠杆菌为供试菌探究了其抑菌机理。结果表明,当V(山苍子精油)∶V(肉桂精油)=2∶1时制备的复配精油对大肠杆菌和金黄色葡萄球菌具有优异的协同抗菌性,抑菌圈直径分别为(21.12±0.17)和(40.26±0.61)mm,且香气较愉悦、舒适、易接受。与单一山苍子精油和肉桂精油相比,复配精油对大肠杆菌的抑制效果更为明显,且可以破坏菌体细胞膜,影响细胞膜通透性,导致细胞内离子、核酸等成分泄漏,使菌液相对电导率明显增加,细菌细胞内容物泄漏程度与单一山苍子精油和肉桂精油相比分别提升140%和20%。In order to solve the problems,such as relatively large dosage,monotonous and strong smell,sensory discomfort and allergic reaction,of single plant essential oil in antibacterial applications,aromatic compound essential oils with synergistic antibacterial effects,based on studies on the antibacterial effects of 13 single essential oil on E.coli and S.aureus,were screened by checkerboard dilution method,antibacterial circle method and sensory evaluation method,and the antibacterial mechanism against E.coli was then investigated.The results showed that compound essential oil with V(litsea cubeba essential oil)∶V(cinnamon essential oil)=2∶1 exhibited excellent synergistic antibacterial activity against both E.coli and S.aureus with inhibition zone diameters(21.12±0.17)and(40.26±0.61)mm,respectively.Meanwhile,the compound essential oil exhibited pleasant and comfortable aroma.Compared with single litsea cubeba essential oil and cinnamon essential oil,the compound essential oils not only showed a more obvious inhibitory effect against E.coli,but also led to bacteria cell membrane damage,increase of cell membrane permeability as well as leakage of ions and nucleic acids,which significantly improved the relative conductivity of bacterial solution.The degree of bacterial cell content leakage caused by compound essential oil was 140% and 20% higher than that by single litsea cubeba essential oil and cinnamon essential oil.

关 键 词：复配植物精油 香气 筛选 协同抗菌作用 抗菌机理 日化原料 

分 类 号：Q946.85[生物学—植物学]