维吾尔族成人急性髓系白血病(非急性早幼粒细胞白血病)骨髓肾母细胞瘤基因1和转录因子ETS-1表达与预后的关系  

Relationship between WT1 and transcription factor ETS-1 expression and prognosis in Uygur adult patients with acute myeloid leukemia(non acute promyelocytic leukemia)

作　　者：阿依姆妮萨·阿卜杜热合曼[1] 姑丽斯坦·阿不都热西提 阿斯亚·麦麦吐逊[3] 努尔比亚·阿布都热西提[1] Ayimunisa-Abudureheman;Gulisitan-Abudourexiti;Asiya-Maymaytituxun;Abudourexiti Nuerbiya(Department of Hematology,The First People's Hospital of Kashi,Kashi,Xinjiang Uygur Autonomous Region 844000,China;Endocrine Department,The First People's Hospital of Kashi,Kashi,Xinjiang Uygur Autonomous Region 844000,China;Breast and Thyroid Surgery,The First People's Hospital of Kashi,Kashi,Xinjiang Uygur Autonomous Region 844000,China)

机构地区：[1]喀什地区第一人民医院血液内科,新疆维吾尔自治区喀什844000 [2]喀什地区第一人民医院内分泌科,新疆维吾尔自治区喀什844000 [3]喀什地区第一人民医院乳腺甲状腺外科,新疆维吾尔自治区喀什844000

出　　处：《安徽医药》2023年第3期466-470,共5页Anhui Medical and Pharmaceutical Journal

基　　金：新疆维吾尔自治区自然科学基金资助项目(2018D01C017)。

摘　　要：目的检测维吾尔族成人急性髓系白血病(非急性早幼粒细胞白血病)[AML(非M3)]病人骨髓肾母细胞瘤基因1(WT1)和转录因子ETS-1表达水平,并探讨二者与AML(非M3)预后的关系。方法选取2015年6月至2018年10月喀什地区第一人民医院血液科收住的105例维吾尔族初诊AML(非M3)病人为观察组。选取同期于该院100例健康体检者为对照组A,及在该院治疗的100例汉族初诊AML(非M3)病人为对照组B。采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测病人骨髓WT1基因和转录因子ETS-1的表达水平;多因素logistic回归分析AML的影响因素;采用Kaplan-Meier法描述生存曲线。结果观察组和对照组B白细胞计数明显高于对照组A(P<0.05),血小板计数及血红蛋白明显低于对照组A(P<0.05);观察组和对照组B病人白细胞计数、血小板计数、血红蛋白比较,差异无统计学意义(P>0.05)。观察组骨髓WT1、ETS-1表达水平分别为0.83±0.15、0.92(0.22,1.23),明显高于对照组B的0.35±0.14、0.48(0.24,1.01)和对照组A的0.01±0.01、0.02(0.01,0.03)(P<0.05),对照组B骨髓WT1、ETS-1表达水平明显高于对照组A(P<0.05)。logistic回归分析显示,WT1、ETS-1均是AML的危险因素;以AML(非M3)病人WT1、ETS-1中位数为界分为低表达组与高表达组,K-M显示WT1、ETS-1低表达组生存率高于高表达组(P<0.05)。结论维吾尔族成人AML(非M3)病人骨髓WT1和ETS-1水平呈高表达,且WT1和ETS-1高表达与疾病的发生及病人预后不良有关。Objective To detect the expression levels of Wilms′ tumor 1 gene(WT1) and transcription factor ETS-1 in patients with acute myeloid leukemia(non acute promyelocytic leukemia) [AML(non-M3)] in Uygur adults, and to explore the relationship between WT1 and ETS-1 and the prognosis of AML(non-M3).Methods One hundred and five Uygur newly diagnosed AML(non-M3) patients admitted to the Hematology Department of The First People’s Hospital of Kashi Region from June 2015 to October 2018 were selected as the observation group, in the same period, 100 healthy subjects in our hospital were selected as control group A, and 100 cases of AML(non-M3) who were treated in our hospital as control group B. The expression levels of WT1 gene and ETS-1 in bone marrow of patients were detected by quantitative Real-time PCR(qRT-PCR), Multi-factor logistic regression analysis was used to analyze the influencing factors of AML;Kaplan Meier(K-M) method was used to describe the survival curve.Results The white blood cell count(WBC) of observation group and control group B were significantly higher than those of control group A(P < 0.05), and the platelet count(PLT) and hemoglobin of observation group and control group B were significantly lower than those of control group A(P < 0.05);there was no significant difference in WBC, PLT, and hemoglobin between the observation group and the control group B(P > 0.05).The expression levels of WT1 and ETS-1 in bone marrow of observation group were(0.83±0.15) and 0.92(0.22, 1.23), respectively,which were significantly higher than those of control group B(0.35±0.14), 0.48(0.24, 1.01) and control group A(0.01±0.01) and 0.02(0.01, 0.03)(P < 0.05). The expression levels of WT1 and ETS-1 in bone marrow of control group B were significantly higher than those of control group A(P < 0.05). Logistic regression analysis showed that WT1 and ETS-1 were risk factors for AML. According to the median of WT1 and ETS-1 in AML(non-M3) patients, they were assigned into low expression group and high expression group.

关 键 词：急性髓系白血病 肾母细胞瘤基因1 转录因子ETS-1 维吾尔族 

分 类 号：R733.71[医药卫生—肿瘤]