新鱼腥草素钠对柑橘指状青霉的抑菌作用  

Antifungal Mechanisms of Sodium New Houttuyfonate Against Penicillium digitatum

作　　者：杜玉玲 杨凡 赵娟 刘书琪 龙超安[1] DU Yuling;YANG Fan;ZHAO Juan;LIU Shuqi;LONG Chaoan(National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,Key Laboratory of Horticultural Plant Biology Ministry of Education,National Citrus Preservation Technology R&D Center,College of Horticultural&Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]果蔬园艺作物种质创新与利用全国重点实验室,园艺植物生物学教育部重点实验室,国家柑橘保鲜技术研发专业中心,华中农业大学园艺林学学院,武汉430070

出　　处：《园艺学报》2023年第1期145-152,共8页Acta Horticulturae Sinica

基　　金：中央高校基本科研业务费专项资金项目(2662020YLPY008);国家现代农业产业技术体系建设专项资金项目(CARS-26);国家自然科学基金项目(32172255,31972122)。

摘　　要：通过离体和活体试验测试新鱼腥草素钠(SNH)对柑橘绿霉病的病原菌指状青霉(Penicillium digitatum)的抑菌效果,最小抑菌浓度和最小杀菌浓度分别为1和2 mmol·L^(-1);随SNH浓度升高,指状青霉孢子萌发率显著下降,相对电导率显著上升;透射电镜结果表明,1 mmol·L^(-1) SNH处理可破坏指状青霉细胞结构,导致细胞质成分溶解,线粒体结构消失,发生质壁分离。同时,1 mmol·L^(-1) SNH可抑制多种柑橘真菌病害的生长,具有广谱抑菌性。采用5、10 mmol·L^(-1) SNH+指状青霉孢子混合液人工接种夏橙果实,其病斑直径显著小于对照。这些结果表明,SNH可显著抑制指状青霉的生长,具有成为柑橘绿霉病防控药剂的前景。In order to explore the antifungal mechanisms of Sodium New Houttuyfonate(SNH)against Penicillium digitatum,in vitro and in vivo experiments were conducted.The results showed that the minimum inhibitory concentration and minimum fungicidal concentration of SNH were 1 and 2 mmol·L^(-1) respectively;with the increase of SNH concentration,the spore germination rate of P.digitatum decreased significantly and the relative conductivity increased significantly.The results of transmission electron microscope showed that 1 mmol·L^(-1) treatment could destroy the cell structure of P.digitatum,resulting in the dissolution of cytoplasmic components,the disappearance of mitochondrial structure and the separation of plasma wall.At the same time,1 mmol·L^(-1) SNH can inhibit the growth of a variety of citrus fungal diseases,showing broad-spectrum antifungal activity.When the fruits were artificially inoculated with 5 and 10 mmol·L^(-1) SNH+Penicillium digitatum spore mixture,the lesion diameter of summer orange was significantly smaller than that of the control group.The above results showed that SNH could significantly inhibit the growth of P.digitatum,and had the prospect of control citrus green mold.

关 键 词：柑橘 采后 绿霉病 生物防治 新鱼腥草素钠 指状青霉 抑菌机制 

分 类 号：S666[农业科学—果树学]