TaABI19 positively regulates grain development in wheat  

作　　者：LIU Yun-chuan WANG Xiao-lu HAO Chen-yang IRSHAD Ahsan LI Tian LIU Hong-xia HOU Jian ZHANG Xue-yong 

机构地区：[1]National Key Facility for Crop Gene Resources and Genetic Improvement,Key Laboratory of Biology and Genetic Improvement of Triticeae Crops,Ministry of Agriculture/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China [2]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,P.R.China [3]Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,P.R.China [4]National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第1期41-51,共11页农业科学学报（英文版）

基　　金：supported by the the Central Public-interest Scientific Institution Basal Research Fund,Chinese Academy of Agricultural Sciences(Y2017PT39).

摘　　要：Starch is the most important component in the endosperm, and its synthesis is regulated by multiple transcription factors(TFs) in cereals. However, whether the functions of these TFs are conserved among cereals remains unclear. In this study,we cloned a B3 family TF in wheat, named TaABI19, based on its orthologous sequence in maize(Zea mays L.). Alignment of the DNA and protein sequences showed that ABI19 is conserved in maize and wheat(Triticum aestivum L.). We found that TaABI19 is highly expressed in young spikes and developing grains, and encodes a nucleus-localized transcriptional activator in wheat. The taabi19-b1 null mutants obtained by EMS exhibited a down-regulation of starch synthesis, shorter grain length and lower thousand-grain weight(TGW). Furthermore, we proved that TaABI19 could bind to the promoters of TaPBF homologous genes and enhance their expression. Haplotype association showed that TaABI19-B1 is significantly associated with TGW. We found that Hap2 and Hap3 were favored and had undergone positive selection in China’s wheat breeding programs. Less than 50% of the modern cultivars convey the favored haplotypes, indicating that TaABI19 still can be considered as a target locus for marker-assisted selection breeding to increase TGW in China.

关 键 词：common wheat transcription factor HAPLOTYPE thousand grain weight 

分 类 号：S512.1[农业科学—作物学]