Characterization of a bla_(CTX-M-3),bla_(KPC-2)and bla_(TEM-1B)co-producing IncN plasmid in Escherichia coli of chicken origin  

作　　者：WANG Wen-jing WANG Yi-fu JIN Ya-jie SONG Wu-qiang LIN Jia-meng ZHANG Yan TONG Xin-ru TU Jian LI Rui-chao LI Tao 

机构地区：[1]Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research,Institute of Pharmacy and Pharmacology,University of South China,Hengyang 421001,P.R.China [2]Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,P.R.China [3]Guilin Medical University,Guilin 541199,P.R.China [4]Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第1期320-324,共5页农业科学学报（英文版）

基　　金：the National Key Research and Development Program of China(2018YFE0192600);the Shanghai Agriculture Applied Technology Development Program,China(T20200104);the Fundamental Research Funds for the Central Universities,China(2020JB05);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202203).

摘　　要：An extensively drug-resistant(XDR)Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui province in China.Genomic analyses indicated that the strain 258E harbors an incompatibility group N(IncN)plasmid pEC258-3,which co-produces bla_(CTX-M-3),bla_(KPC-2),bla_(TEM-1B),qnrS1,aac(6')-Ib-cr,dfrA14,arr-3,and aac(6')-Ib3.Multiple genome arrangement analyses indicated that pEC258-3 is highly homologous with pCRKP-1-KPC discovered in Klebsiella pneumoniae from a patient.Furthermore,conjugation experiments proved that plasmid pEC258-3 can be transferred horizontally and may pose a significant potential threat in animals,community and hospital settings.

关 键 词：bla_(CTX-M-3) bla_(KPC-2) bla_(TEM-1B) IncN PLASMID Escherichia coli 

分 类 号：S852.61[农业科学—基础兽医学]