迷迭香酸对大鼠急性脊髓损伤的神经保护作用及其机制  

Neuroprotective Effect of Rosmarinic Acid on Acute Spinal Cord Injury in Rats and Its Mechanism

作　　者：李丰 周文华[1] 陈大勇 刘超[1] 付军初 潘丹 谭志红 唐少龙[1] 黄庆华[1] LI Feng;ZHOU Wenhua;CHEN Dayong;LIU Chao;FU Junchu;PAN Dan;TAN Zhihong;TANG Shaolong;HUANG Qinghua(Department of Spinal Surgery,Zhuzhou Hospital,Xiangya School of Medicine,Central South University,Zhuzhou 412000,Hunan China.)

机构地区：[1]中南大学湘雅医学院附属株洲医院脊柱外科,湖南株洲412000

出　　处：《中国中医骨伤科杂志》2023年第2期1-5,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：目的:探究迷迭香酸(RA)对大鼠急性脊髓损伤(ASCI)的神经保护作用及其机制。方法:50只SD大鼠随机分为5组,即假手术组(Sham组)、急性脊髓损伤组、迷迭香酸低、中、高剂量组(RA-L、RA-M、RA-H组),除Sham组外均建立大鼠急性脊髓损伤模型,造模成功后,Sham组和急性脊髓损伤组灌胃等量生理盐水,而RA-L组、RA-M组、RA-H组灌胃5,20,40 mg/kg剂量迷迭香酸。在给药后第3,7,14,28天进行BBB评分;ELISA法检测脊髓损伤组织SOD、GSH-PX、MDA、TNF-α、IL-1β、IL-10、NO;Western Blot法检测脊髓损伤组织iNOS、BDNF、p-NF-κB、p-p38 MAPK蛋白表达。结果:与Sham组比较,急性脊髓损伤组造模后第3,7,14,28天后BBB评分降低;与急性脊髓损伤组比较,给药第3,7,14,28天后,RA-L组、RA-M组、RA-H组BBB评分升高。与Sham组比较,急性脊髓损伤组SOD、GSH-PX活性降低,MDA含量、TNF-α、IL-1β、IL-10、NO含量增加,iNOS、BDNF、p-NF-κB、p-p38 MAPK蛋白表达水平上调;与急性脊髓损伤组比较,RA-L组、RA-M组、RA-H组SOD、GSH-PX活性升高,MDA含量、TNF-α、IL-1β、IL-10、NO含量降低,iNOS、BDNF、p-NF-κB、p-p38 MAPK蛋白表达水平下调。结论:迷迭香酸可能通过抑制NF-κB、p38 MAPK信号通路减轻大鼠急性脊髓损伤组织氧化应激反应及炎症反应,对大鼠急性脊髓损伤的神经有保护作用。Objective:To investigate the neuroprotective effect and mechanism of rosmarinic acid(RA)on acute spinal cord injury(ASCI)in rats.Methods:In the study,50 SD rats were randomly divided into Sham operation group(Sham group),ASCI group,RA low,medium and high dose groups(RA-L,RA-M,RA-H group).Except for sham group,ASCI models were established in rats.After successful modeling,the Sham group and ASCI group were given the same amount of normal saline,while the RA-L group,RA-M group and RA-H group were given 5,20,and 40 mg/kg RA.BBB score was performed on the day 3,day 7,day 14 and day 28 after administration.ELISA was used to detect SOD,GSH-PX,MDA,TNF-α,IL-1β,IL-10 and NO in spinal cord injury tissue.Western Blot was used to detect the protein expression of iNOS,BDNF,p-NF-κB and p-p38 MAPK in injured tissue.Results:Compared with the Sham group,the BBB score of the ASCI group decreased at day 3,day 7,day 14,and day 28 after administration.Compared with the ASCI group,the RA-L group,RA-M group,RA-H group BBB score increased at day 3,day 7,day 14,and day 28 after administration.Compared with the Sham group,the activities of SOD and GSH-PX in the ASCI group decreased,the contents of MDA,TNF-α,IL-1β,IL-10 and NO increased,and the protein expressions of iNOS,BDNF,p-NF-κB and p-p38 MAPK were up-regulated.Compared with the ASCI group,the activities of SOD and GSH-PX in the RA-L group,RA-M group and RA-H group were increased,and the contents of MDA,TNF-α,IL-1β,IL-10 and NO were decreased.The protein expression levels of BDNF,p-NF-κB and p-p38 MAPK were down-regulated.Conclusion:RA may reduce oxidative stress and inflammatory response in rat ASCI spinal cord injury tissues by inhibiting NF-κB and p38 MAPK signaling pathways,and has a protective effect on the nerves of rat ASCI.

关 键 词：迷迭香酸 急性脊髓损伤 神经保护 氧化应激反应 炎症反应 

分 类 号：R-33[医药卫生]