miR-367-3p调控细胞周期素D2重组蛋白表达对肺癌细胞增殖、迁移的影响  

The effects of miR-367-3p regulating CCND2 expression on the proliferation and migration of lung cancer cells

作　　者：曹磊 张成立 侯颖 CAO Lei;ZHANG Chengli;HOU Ying(Department of Cardiothoracic Surgery,the First Affiliated Hospital of Shaoyang University,Hunan,Shaoyang 422000,China)

机构地区：[1]湖南省邵阳学院附属第一医院心胸外科,422000

出　　处：《临床外科杂志》2022年第12期1123-1127,共5页Journal of Clinical Surgery

摘　　要：目的探讨miR-367-3p调控细胞周期素D2重组蛋白(CCND2)表达对肺癌细胞增殖、迁移的影响。方法选取人肺癌细胞Calu、MSTO-211H、NCI-H2347、NCI-H1437、NCI-H1944、NCI-H647、人正常肺细胞HLF-a,qRT-PCR法测定细胞中miR-367-3p表达水平;将Calu细胞分为对照组、miR-367-3p NC组、miR-367-3p mimic组、miR-367-3p mimic+pc-CCND2组;CCK-8法测定Calu细胞活力;流式细胞仪测定细胞凋亡能力;Transwell实验测定细胞侵袭能力;划痕实验测定Calu细胞迁移能力;WB法测定Calu细胞CCND2及凋亡、侵袭相关蛋白表达;荧光素酶实验测定miR-367-3p与CCND2的靶向关系。结果miR-367-3p在人肺癌细胞中呈现低表达;与对照组比较,miR-367-3p mimic组Calu细胞活力、侵袭、迁移能力、MMP-2、MMP-9蛋白表达显著降低,凋亡能力、Caspase-3、Bax蛋白表达显著升高,Bcl-2蛋白表达显著降低,差异有统计学意义(P<0.05);CCND2过表达可逆转miR-367-3p过表达对Calu细胞活力、侵袭、迁移能力的抑制作用。荧光素酶实验结果显示,miR-367-3p与CCND2存在靶向调节关系。结论miR-367-3p可抑制人肺癌细胞Calu的增殖及迁移能力,可能是通过抑制CCND2实现的。Objective To investigate the effect of miR-367-3p regulating the expression of recombinant cyclin D2 protein(CCND2)on the proliferation and migration of lung cancer cells.Methods Human lung cancer cells Calu,MSTO-211H,NCI-H2347,NCI-H1437,NCI-H1944,NCI-H647,human normal lung cells HLF-a were selected,qRT-PCR method was used to determine the expression level of miR-367-3p in the cells.Calu cells were divided into control group,miR-367-3p NC group,miR-367-3p mimic group,miR-367-3p mimic+pc-CCND2 group,CCK-8 method was used to determine the viability of Calu cells in each group;flow cytometry was used to measure cell apoptosis ability.Transwell test was used to measure cell invasion ability.Scratch test was used to measure the migration ability of Calu cells.WB method was used to determine the relative expression of CCND2,apoptosis and invasion-related proteins in Calu cells.Luciferase assay was used to determine the targeting relationship between miR-367-3p and CCND2.Results MiR-367-3p showed low expression in human lung cancer cells;compared with the control group,the Calu cell viability,invasion,migration ability,MMP-2 and MMP-9 protein expression in the miR-367-3p mimic group were significantly reduced(P<0.05),the apoptosis ability,Caspase-3,Bax protein expression were significantly increased(P<0.05),the Bcl-2 protein expression was significantly reduced(P<0.05);CCND2 overexpression reversed the inhibitory effect of miR-367-3p overexpression on Calu cell viability,invasion,and migration;the results of luciferase experiments showed that miR-367-3p and CCND2 had a targeted regulatory relationship.Conclusion MiR-367-3p can inhibit the proliferation and migration of human lung cancer cells Calu,which may be achieved by inhibiting CCND2.

关 键 词：miR-367-3p 细胞周期素D2重组蛋白 肺癌 增殖 迁移 

分 类 号：R734.2[医药卫生—肿瘤]