高效液相色谱法同时测定校园及周边市售辣条中多种合成色素含量  

Simultaneous determination of 9 synthetic pigments in spicy strip on campus and around by high performance liquid chromatography

作　　者：朱小琼[1] 王永根[1] 石仁其 姚凤燕[1] 包玲月 王剑波[1] ZHU Xiao-qiong;WANG Yong-gen;SHI Ren-qi;YAO Feng-yan;BAO Ling-yue;WANG Jian-bo(Pinghu Municipal Center for Disease Control and Prevention,Zhejiang 314200,China)

机构地区：[1]平湖市疾病预防控制中心,浙江314200

出　　处：《中国卫生检验杂志》2023年第3期282-285,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的 建立同时测定辣条中多种合成色素含量(柠檬黄、新红、苋菜红、胭脂红、日落黄、诱惑红、红2G、亮蓝、赤藓红)的高效液相色谱法。方法 样品采用正己烷去油脂,α-淀粉酶、木瓜蛋白酶按试样质量分数2%、2.5%的比例相混合,水浴65℃,反应15 min,去除淀粉、蛋白等杂质后,用10%氨化甲醇提取合成色素,再用固相萃取小柱净化,进行高效液相色谱分析;为避免假阳性样品的出现,同时以各自目标峰的出峰时间和特征波长进行双定性。结果 9种色素浓度在1.0μg/ml~30.0μg/ml内线性关系良好,相关系数(r)≥0.999。方法检出限为0.08μg/ml~0.27μg/ml,回收率为81.2%~100.0%,相对标准偏差为0.14%~2.8%(n=6)。结论 本方法重现性好、准确度高,可同时测定辣条样品中9种人工合成色素,并应用于平湖市校园及周边市售辣条中多种合成色素含量的测定。Objective A high performance liquid chromatography(HPLC) method was developed for the simultaneous determination of lemon yellow, new red, amaranth, carmine, sunset yellow, allura red, red 2G, light blue, etythrosine in spicy strip. Methods The samples were degreased with n-hexane, mixed with α-amylase and papain at the ratio of 2% and 2.5%, and then reacted in a water bath at 65 ℃ for 15 min. After removing impurities such as starch and protein, synthetic pigments were extracted with 10% ammoniated methanol, and then purified by solid phase extraction column for HPLC analysis. In order to avoid the occurrence of false positive samples, the peak time and characteristic wavelength of each target peak were used for bilateralization. Results The linear relationship was good in the range of 1.0 μg/ml to 30.0 μg/ml with correlation coefficient(r) ≥0.999. The detection limits were within 0.08 μg/ml-0.27 μg/ml, the recoveries were within 81.2%-100.0%, and the relative standard deviations(RSD) were 0.14%-2.8%(n=6). Conclusion This method has good reproducibility and high accuracy, which can simultaneously determine 9 synthetic pigments and be applied to the determination of various synthetic pigments in spicy strip in Pinghu campus and surrounding markets.

关 键 词：高效液相色谱法 合成色素 辣条 

分 类 号：O657.72[理学—分析化学]