不同分子量海蚌类肝素理化性质分析及抗血栓活性评价  

Physicochemical analysis and evaluation of the antithrombotic activity of different molecular weights of heparin from clam Coelomactra antiquata

作　　者：陈观兰 陈建平[1] 李瑞[1] 贾学静 刘晓菲 宋兵兵 钟赛意[1,2,3] CHEN Guanlan;CHEN Jianping;LI Rui;JIA Xuejing;LIU Xiaofei;SONG Bingbing;ZHONG Saiyi(Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Guangdong Provincial Engineering Technology Research Center of Seafood(College of Food Science and Technology,Guangdong Ocean University),Zhanjiang 524008,China;Shenzhen Research Institute,Guangdong Ocean University,Shenzhen 518108,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,China)

机构地区：[1]广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心(广东海洋大学食品科技学院),广东湛江524008 [2]广东海洋大学深圳研究院,广东深圳518108 [3]海洋食品精深加工关键技术省部共建协同创新中心(大连工业大学),辽宁大连116034

出　　处：《热带海洋学报》2023年第1期152-160,共9页Journal of Tropical Oceanography

基　　金：广东省重点领域研发计划项目(2020B1111030004);国家重点研发计划重点专项(2019YFD0902005);深圳市科技计划项目(JCYJ20170818111335796、PT202001-17);湛江市科技计划项目(2019A01015);广东省高校科技创新团队项目(2021KCXTD021)。

摘　　要：为探究不同分子量海蚌类肝素的理化性质、体内抗血栓活性及对出血时间的影响,本文以海蚌类肝素G2(60.25kDa)及其2种不同分子量的降解产物DG1(24.48kDa)、DG2(6.75kDa)为研究对象,首先采用改良的硫酸-咔唑法和BaCl_(2)-gel比浊法测定糖醛酸及硫酸基的含量,利用纳米粒径仪测定粒径、Zeta电位,并同步利用热分析仪分析热稳定性;然后通过小鼠黑尾模型和小鼠断尾模型分别研究抗血栓活性及对出血时间的影响。研究结果表明,随着分子量的降低,海蚌类肝素硫酸基含量升高,糖醛酸含量降低,初始降解温度先升高后降低,粒径降低,Zeta电位升高。相对于海蚌类肝素G2,其适度降解产物DG1具有更高的抑制尾部血栓形成的能力,同时具有较低的出血风险。To investigate the physicochemical properties in vivo antithrombotic activity and their effects on the bleeding time of different molecular weights of clam heparinoid,native clam heparinoid G2(60.25 kDa),and its two different degradation products,DG1(24.48 kDa)and DG2(6.75 kDa),were taken as the research objects in this study.The content of glucuronic acid and the sulfate group was respectively determined by the modified sulfuric acid-carbazole method and BaCl_(2)-gel turbidimetric method,the particle size and zeta potential were determined by nanoparticle sizer,and the thermal stability was analyzed by the simultaneous thermal analyzer.Then the antithrombotic activity and the effect on bleeding time were investigated by the mice black tail model and mice broken tail model,respectively.The results showed that as the molecular weight of clam heparinoid decreased,the sulfate content increased,the glucuronic acid content decreased,the initial degradation temperature increased and then decreased,the particle size decreased,and the Zeta potential increased.The moderate degradation product of G2,DG1,has a higher ability to inhibit tail thrombosis with a lower risk of hemorrhage compared to the natural clam heparinoid G2.

关 键 词：海蚌类肝素 理化性质 抗血栓 出血副作用 

分 类 号：P745.3[天文地球—海洋生物学]