牙本质基质蛋白1糖基化形式在颌骨损伤中的作用  

Role of proteoglycan form of dentin matrix protein 1 in jaw defects

作　　者：刘洋 李剑奎 沙伟君 崔立然 王辉 徐浩[2] 张怀胜 宋波[2] 薛徽 LIU Yang;LI Jiankui;SHA Weijun;CUI Liran;WANG Hui;XU Hao;ZHANG Huaisheng;SONG Bo;XUE Hui(the First Affiliated Hospital of Qiqihaer Medical University,Qiqihaer 161041,China;Qiqihaer Medical University,Qiqihaer 161006)

机构地区：[1]齐齐哈尔医学院附属第一医院,黑龙江齐齐哈尔161041 [2]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《中国比较医学杂志》2022年第12期16-23,共8页Chinese Journal of Comparative Medicine

基　　金：黑龙江省自然科学基金资助项目(LH2020H128);黑龙江省省属高等学校基本科研业务费科研项目(2019-KYYWF-1218)。

摘　　要：目的探讨牙本质基质蛋白1糖基化形式(proteoglycan form of dentin matrix protein 1,DMP1-PG)在下颌骨骨损伤中的重要作用。方法分别构建对照组野生型(wild type,WT)小鼠及实验组牙本质基质蛋白1糖基化点突变(S89G-DMP1)小鼠下颌骨骨缺损模型。模型构建完成后7 d、14 d及28 d收取样本,显微计算机断层micro-CT扫描,组织切片及染色,观察二者之间组织愈合差异;通过免疫荧光染色,观察成骨相关蛋白在组织损伤区的表达变化差异;应用实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)技术,对比实验组与对照组在术后成骨相关基因表达变化情况;分别提取实验组与对照组颌骨骨髓间充质干细胞,进行成骨诱导分化,观察二者之间成骨分化能力差异。结果同对照组相比,实验组小鼠下颌骨损伤愈合能力明显减弱,骨损伤区成骨相关蛋白表达减低,术后7 d及14 d成骨相关基因表达水平较对照组明显下调。通过提取两组小鼠下颌骨骨髓间充质干细胞进行成骨诱导分化,结果显示同对照组相比,实验组小鼠骨髓间充质干细胞成骨向分化能力明显减弱。结论DMP1-PG参与颌骨损伤修复,缺乏DMP1-PG可导致小鼠颌骨缺损愈合延迟。Objective To investigate the role of proteoglycan form of dentin matrix protein 1(DMP1-PG)in mandibular bone injury.Methods We created mandibular bone defects in wild-type mice(control group)and DMP1 glycosylation point mutant(S89G-DMP1)mice(experimental group).Samples of mandibles were collected at 7,14 and 28 days after model construction and subjected to micro-computed tomography scans,tissue sectioning,and hematoxylin eosin and toluidine blue staining to compare defect healing between the two groups.We also examined differences in the expression of osteogenesis-related proteins in the injured areas by immunofluorescence staining,and compared osteogenesis-related gene expression levels between the experimental and control groups post-surgery using real-time quantitative polymerase chain reaction.Mandibular bone marrow mesenchymal stem cells were extracted from both groups and their osteogenic differentiation abilities were compared.Results Mandibular injury healing was significantly reduced in the experimental compared with the control mice,and expression levels of osteogenesis-related proteins in the bone-injury area were decreased.In addition,the osteogenic differentiation ability of bone marrow mesenchymal stem cells from the experimental group was significantly weaker than that of cells from the control group.Conclusions DMP1-PG is involved in the repair of jaw injury,and lack of DMP1-PG can lead to delayed healing of jaw defects in mice.

关 键 词：颌骨缺损 蛋白聚糖 牙本质基质蛋白1 

分 类 号：R-33[医药卫生]