缺血时间差异对大鼠肠缺血再灌注损伤的影响研究  

Effects of ischemia time on intestinal ischemia-reperfusion injury in a rat model

作　　者：潘晓慧 张朋辉 江国健 李娟 陈桂煌 姚欣伶 冯学轩 PAN Xiaohui;ZHANG Penghui;JIANG Guojian;LI Juan;CHEN Guihuang;YAO Xinling;FENG Xuexuan(Guangdong Medical Laboratory Animal Center,Guangzhou 528248,China)

机构地区：[1]广东省医学实验动物中心,广州528248

出　　处：《中国比较医学杂志》2022年第12期32-36,共5页Chinese Journal of Comparative Medicine

基　　金：广东省中医药局科研项目(20211056);广东省医学科学技术研究基金项目(A2021396)。

摘　　要：目的从氧化损伤途径及组织病理学层面评价小肠缺血时间对小肠缺血再灌注模型的影响,为该疾病的研究提供建模条件及基础数据。方法24只雄性SD大鼠随机分为假手术组、缺血30 min组、缺血1 h组、缺血2 h组,各造模组结扎肠系膜上动脉使缺血一定时间后,再灌注4 h诱导缺血再灌注损伤模型。恢复供血4 h后大鼠安乐死,取10%肠组织匀浆后通过试剂盒对应检测,比较小肠组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)水平,HE染色观察肠组织形态,并测量小肠绒毛高度及黏膜厚度。结果与假手术组比较,缺血30 min组及缺血1 h组的SOD、GSH-Px、MDA水平显著升高(P<0.05),缺血30 min、1 h及2 h均可使炎性细胞浸润加重,病理Chiu’s评分显著升高(P<0.05),此外,缺血1 h及2 h还可显著降低小肠绒毛高度及黏膜厚度(P<0.01)。但缺血2 h可引起动物死亡(死亡率33.3%)。结论缺血1 h再灌注4 h建立的小肠缺血再灌注模型的氧化指标及肠道组织形态最为理想,该条件下建立的小肠缺血再灌注模型适用于疾病研究及药物开发。Objective To evaluate the effects of ischemia time on intestinal ischemia-reperfusion injury in a rat model by assessing oxidative damage and histopathology,to provide optimal modeling conditions and basic data for future studies.Methods Twenty-four male SD rats were divided randomly into a sham operation group,30 min ischemia group,1 h ischemia group and 2 h ischemia group.Ischemia-reperfusion injury models were induced by ischemia of the superior mesenteric artery for the noted time,followed by reperfusion for 4 h.After resumption of the blood supply,the rats were euthanized.Than,take 10%intestinal tissue homogenate and test with kit,test the levels of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)and glutathione were determined.The morphology of the intestinal tissue was observed by hematoxylin and eosin staining,and villus height and mucosa thickness were measured.Results SOD,GSH-Px and MDA were all significantly increased in the 30 min and 1 h ischemia groups compared with the sham operation group(P<0.05).Inflammatory cell infiltration was aggravated and the pathological Chiu’s score was significantly increased in the ischemia groups(P<0.05).In addition,1 h and 2 h of ischemia significantly reduced the villus height and mucosal thickness in the small intestine(P<0.01),while 2 h of ischemia could cause death(death rate 33.3%).Conclusions An intestinal ischemia-reperfusion model established by 1 h ischemia and 4 h reperfusion had the most ideal oxidation index and intestinal tissue morphology,and is thus suitable for disease research and drug development.

关 键 词：小肠缺血再灌注损伤 缺血时间 氧化指标 组织病理学 

分 类 号：R-33[医药卫生]