适宜肉鸭全价料发酵的益生菌体外筛选  

Probiotics which suitable for the fermentation of full price meat duck were screened in vitro

作　　者：亓秀晔 谢全喜[1] 刘乃芝[1] 郭杨丽 徐海燕[1] QI Xiuye;XIE Quanxi;LIU Naizhi;GUO Yangli;XU Haiyan(Shandong BaoLai-LeeLai BioengineeringCo.,Ltd.,Shandong Key Laboratory of Animal Microecological Preparations,Tai’an,Shandong Province 271000,China)

机构地区：[1]山东宝来利来生物工程股份有限公司山东省动物微生态制剂重点实验室,山东泰安271000

出　　处：《中国饲料》2023年第5期121-125,共5页China Feed

摘　　要：本试验利用乳酸杆菌和酵母菌体外发酵肉鸭全价料,通过发酵前后活菌数、pH、粗蛋白质、酸溶蛋白、总酸含量等指标的比较,筛选出适宜肉鸭全价料发酵的乳酸杆菌和酵母菌。结果表明:(1)适宜肉鸭全价料发酵的最适乳酸杆菌为BLCC2-0111和BLCC2-0112,发酵至72 h时,pH达到最低,分别为4.03和4.13,活菌数最高,分别为33.61×10^(8)CFU/g和25.68×10^(8)CFU/g。(2)适宜肉鸭全价料发酵的最适酵母菌为BLCC4-0050和BLCC4-0021,发酵至72 h时,粗蛋白质含量最高,分别为17.78%和17.77%,活菌数最高,分别为30.23×10^(8)CFU/g和35.54×10^(8)CFU/g。(3)适宜肉鸭全价料发酵的最适混菌组合为BLCC2-0111+BLCC4-0021,发酵至72 h时,pH降至最低4.12,乳酸杆菌活菌数38.00×10^(8)CFU/g,总酸含量20.61 mg/g,酸溶蛋白含量13.49%,混菌发酵的饲料营养价值显著优于单菌发酵。In this experiment,Lactobacillus and yeast were used to ferment the whole meat duck in vitro.Through the comparison of the number of live bacteria,pH,crude protein,acid soluble protein and total acid content before and after fermentation,the Lactobacillus and yeast suitable for the fermentation of the whole meat duck were selected.The results were as follows:(1)BLCC2-0111 and BLCC2-0112 were the most suitable Lactobacillus for the fermentation of full price meat duck.After 72 h of fermentation,the pH reached the lowest,4.03 and 4.13 respectively,and the number of viable bacteria was the highest,33.61×10^(8)CFU/g and 25.68×10^(8)CFU/g respectively.(2)BLCC4-0050 and BLCC4-0021were the most suitable yeasts for the fermentation of full price meat duck.After72 h of fermentation,the content of crude protein was the highest,17.78%and 17.77%respectively,and the number of viable bacteria was the highest,30.23×10^(8)CFU/g and35.54×10^(8)CFU/g respectively.(3)The optimum mixed bacteria combination suitable for the fermentation of full price meat duck was BLCC2-0111+BLCC4-0021.When fermented to 72 h,the pH decreased to the lowest of4.12 and the number of live Lactobacillus was 38.00×10^(8)CFU/g,total acid content 20.61 mg/g and acid soluble protein content 13.49%.The feed nutritional value of mixed bacteria fermentation was significantly better than that of single bacteria fermentation.

关 键 词：肉鸭全价料 体外筛选 乳酸杆菌 酵母菌 混菌发酵 

分 类 号：S816.6[农业科学—饲料科学]