宫颈局部IFI16、STAT1蛋白、T-bet蛋白表达与HPV16/18感染转归相关性及联合检测价值分析  

Correlation between local IFI16,STAT1 protein and T-bet protein expression and regression of HPV16/18 infection in the uterine cervix and analysis of the value of combined detection

作　　者：潘晓伟 史鹏飞 任海霞 陈何伟 王宏卫 PAN Xiaowei;SHI Pengfei;REN Haixia;CHEN Hewei;WANG Hongwei(Department of Gynecology,Shijiazhuang People′s Hospital,Shijiazhuang,Hebei 050011,China)

机构地区：[1]石家庄市人民医院妇科,河北石家庄050011

出　　处：《国际检验医学杂志》2023年第5期594-598,共5页International Journal of Laboratory Medicine

基　　金：河北省2021年度医学医学科学研究课题(20210998)。

摘　　要：目的探讨宫颈局部干扰素诱导蛋白16(IFI16)、信号传导和转录激活因子1(STAT1)蛋白、T-box基因家族的新型转录因子T-bet蛋白表达与人乳头瘤病毒(HPV)16/18感染转归相关性及联合检测价值。方法选取2020年5月至2021年12月该院收治的110例人乳头瘤病毒(HPV)16/18感染患者,均根据病情并结合患者意愿给予药物、高强度聚焦超声(HIFU)、宫颈环形电切术(LEEP)治疗,统计3种方法治疗后的转阴率,并根据治疗后HPV16/18感染转归情况,分为转阴组(60例)、未转阴组(50例)。比较两组宫颈局部IFI16、STAT1蛋白、T-bet蛋白表达,多因素Logistic回归分析HPV16/18感染转归的相关影响因素,受试者工作特征曲线及曲线下面积(AUC)分析IFI16、STAT1蛋白、T-bet蛋白及联合检测预测HPV16/18感染转归价值。结果转阴组采取HIFU、LEEP治疗方法的患者占比与采取药物治疗方法的患者占比比较,差异有统计学意义(P<0.05);未转阴组IFI16、STAT1蛋白表达高于转阴组,T-bet蛋白表达低于转阴组,差异有统计学意义(P<0.05);多因素Logistic回归分析显示,阴道炎、治疗方法、IFI16、STAT1蛋白、T-bet蛋白表达均与HPV16/18感染转归有关(P<0.05);IFI16、STAT1蛋白、T-bet蛋白联合检测预测HPV16/18感染未转阴的AUC最大,三者联合检测的灵敏度为88.00%,特异度为85.00%;IFI16、STAT1蛋白高表达患者转阴率低于IFI16、STAT1蛋白低表达患者,T-bet蛋白高表达患者转阴率高于T-bet蛋白低表达患者,差异有统计学意义(P<0.05)。结论宫颈局部IFI16、STAT1蛋白、T-bet蛋白表达与HPV16/18感染转归有关,三者联合检测可作为预测HPV16/18感染转归的一个可靠方案,在保证转阴率的同时,又避免过度治疗增加患者负担。Objective To investigate the correlation between the expression of cervical local interferon-inducible protein 16(IFI16),signal transducer and activator of transcription 1(STAT1)protein,and T-bet protein,a novel transcription factor of the T-box gene family,and the regression of human papillomavirus(HPV)16/18 infection and the value of the combined assay.Methods A total of 110 patients with HPV16/18 infection admitted to a hospital from May 2020 to December 2021 were selected,and they were treated with drugs,high-intensity focused ultrasound(HIFU),and loop electrosurgical excision(LEEP)according to their conditions and patients′wishes,the rate of negative conversion after treatment by the three methods was counted,and according to the outcome of HPV16/18 infection after treatment,they were divided into negative conversion group(60 cases)and non-negative conversion group(50 cases).The local expressions of IFI16,STAT1 protein and T-bet protein were compared between the two groups,and the relevant influencing factors of the outcome of HPV16/18 infection were analyzed by multivariate Logistic regression.Receiver operating characteristic curve and area under curve(AUC)were used to analyze IFI16,STAT1 protein,T-bet protein and their combined detection predictive value for the outcome of HPV16/18 infection.Results There was a statistically significant difference between the proportion of patients who took HIFU and LEEP treatment and the proportion of patients who took drug treatment in the conversion group(P<0.05).The expression of IFI16 and STAT1 protein in non-negative group was higher than that in negative group,while the expression of T-bet protein was lower than that in negative group,and the difference was statistically significant(P<0.05).Multiple Logistic regression analysis showed that vaginitis,treatment,IFI16,STAT1 protein and T-bet protein expression were correlated with the outcome of HPV16/18 infection(P<0.05).The combination of IFI16,STAT1 protein,and T-bet protein had the largest AUC for predicting non

关 键 词：人乳头瘤病毒16/18 干扰素诱导蛋白16 信号传导和转录激活因子1 转录因子 感染转归 

分 类 号：R737.33[医药卫生—肿瘤]